Research code: IR.UMZ.REC.097004
Ethics code: IR.UMZ.REC.097004
Clinical trials code: IR.UMZ.REC.097004
1- Farhangian University, Nasibah Campus, Tehran,, Farhangian University, Nasibah Campus, Tehran,
2- Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Mazandaran, Iran, Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Mazandaran, Iran ,mousavi2753@gmail.com
2- Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Mazandaran, Iran, Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Mazandaran, Iran ,
Abstract: (10 Views)
Background: Multiple sclerosis is an autoimmune disease with inflammatory and demyelinating lesions in the central nervous system. The aim of this study was to assessing the response of Neuro-Inflammatory Factors in brain Tissue in rats with experimental autoimmune encephalomyelitis to the protective effect of interval training and vitamin D.
Material and method: In this experimental study, 48 male Louise 4-week-old female rats were randomly divided into 8 groups. The interval protocol consisted of 10 repetitions of 1 minute, the ratio of work to rest was 1:2, and the total running time was 30 minutes. The training intensity was gradually increased from 25 meters per minute to 70 meters per minute. The rats were immunized with the guinea pig's spit and complete adjuvant. The EAE model was induced at the end of the sixth week of exercise. Rats received 5 μg of body weight per kilogram of vitamin D3 in 150 μl of sesame oil every two days once for 2 weeks’ intraperitoneal injection. Data were analyzed by one-way analysis of variance and Tukey post hoc test at the p <0.05.
Results: The results showed that there was no significant difference between the groups in NF-KB and TGF-B (p>0.05). The average changes of IL-17 in the vitamin D group in the EAE model had significant increase compared to the healthy vitamin D control group (P=0.01). The average changes of GM-CSF in healthy interval groups (P=0.018), D healthy control (P=0.02), D healthy interval (P=0.001) and D interval MS (P=0.03) There was significant decrease compared to the healthy control group.
Conclusion: interval training and vitamin D were not associated with the improvement of brain neuroinflammatory factors in rats with experimental autoimmune encephalomyelitis.
Material and method: In this experimental study, 48 male Louise 4-week-old female rats were randomly divided into 8 groups. The interval protocol consisted of 10 repetitions of 1 minute, the ratio of work to rest was 1:2, and the total running time was 30 minutes. The training intensity was gradually increased from 25 meters per minute to 70 meters per minute. The rats were immunized with the guinea pig's spit and complete adjuvant. The EAE model was induced at the end of the sixth week of exercise. Rats received 5 μg of body weight per kilogram of vitamin D3 in 150 μl of sesame oil every two days once for 2 weeks’ intraperitoneal injection. Data were analyzed by one-way analysis of variance and Tukey post hoc test at the p <0.05.
Results: The results showed that there was no significant difference between the groups in NF-KB and TGF-B (p>0.05). The average changes of IL-17 in the vitamin D group in the EAE model had significant increase compared to the healthy vitamin D control group (P=0.01). The average changes of GM-CSF in healthy interval groups (P=0.018), D healthy control (P=0.02), D healthy interval (P=0.001) and D interval MS (P=0.03) There was significant decrease compared to the healthy control group.
Conclusion: interval training and vitamin D were not associated with the improvement of brain neuroinflammatory factors in rats with experimental autoimmune encephalomyelitis.
Type of Study: Research |
Subject:
Exercise Physiology
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