Volume 29, Issue 11 (1-2023)
RJMS 2023, 29(11): 0-0 |
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جلالیان ف, نصیرزاده ونهری ا. The effect of Conotoxin (snail venom) on Bax and P53 gene expression in Mcf7 breast cancer cells. RJMS 2023; 29 (11)
URL: http://rjms.iums.ac.ir/article-1-7183-en.html
URL: http://rjms.iums.ac.ir/article-1-7183-en.html
Abstract: (289 Views)
Background and Aim: Breast cancer is the second leading cause of death in women after lung cancer. Many genes along with different environmental factors are involved in the development of breast cancer, each of which plays an important role in increasing the risk of this type of disease in women. In this study, the effect of canatoxin toxin on the expression of Bax and P53 genes, which are key genes in the apoptosis cycle in the MCF-7 cancer line, was investigated.
Materials and Methods: In this study, MCF-7 breast cancer cells were cultured in RPMI medium with 10% bovine embryos. After culture and growth, the cells received the toxin toxin toxin and in two times (48 and 72 hours) the expression of p53 and Bax genes in the treated and control cells was examined using the Real time RT_ PCR technique. Took. Statistical analysis was performed using SPSS software version 22. The difference in gene expression between healthy control (control) and patient groups was used using t-test (T test) and the difference between different groups with P-value of 0.05 was considered significant.
Results: According to the statistical analysis after 48 hours of lipofect, the toxin had no significant effect on the expression of p53 (P=0.94) and (P=0.19) Bax genes in treated cells, but After 72 hours after lipofect, the toxin significantly reduced the expression of Bax gene (P=0.01) and significantly increased the expression of p53 gene (P=0.038).
Conclusion: According to previous studies, Bax gene in Mcf7 cancer cell has increased gene expression and has anti-apoptotic activity. Due to cannatoxin toxin, gene expression is reduced and the process of cell death is regular. Also, P53 gene, which is a tumor suppressor in the apoptosis cycle, had a significant decrease in expression in Mcf7 cancer cells and an increase in expression was observed due to cantotoxin toxin. As a result, it can be said that cantotoxin toxin can activate the apoptotic cycle and prevent cancer from progressing with planned death.
Materials and Methods: In this study, MCF-7 breast cancer cells were cultured in RPMI medium with 10% bovine embryos. After culture and growth, the cells received the toxin toxin toxin and in two times (48 and 72 hours) the expression of p53 and Bax genes in the treated and control cells was examined using the Real time RT_ PCR technique. Took. Statistical analysis was performed using SPSS software version 22. The difference in gene expression between healthy control (control) and patient groups was used using t-test (T test) and the difference between different groups with P-value of 0.05 was considered significant.
Results: According to the statistical analysis after 48 hours of lipofect, the toxin had no significant effect on the expression of p53 (P=0.94) and (P=0.19) Bax genes in treated cells, but After 72 hours after lipofect, the toxin significantly reduced the expression of Bax gene (P=0.01) and significantly increased the expression of p53 gene (P=0.038).
Conclusion: According to previous studies, Bax gene in Mcf7 cancer cell has increased gene expression and has anti-apoptotic activity. Due to cannatoxin toxin, gene expression is reduced and the process of cell death is regular. Also, P53 gene, which is a tumor suppressor in the apoptosis cycle, had a significant decrease in expression in Mcf7 cancer cells and an increase in expression was observed due to cantotoxin toxin. As a result, it can be said that cantotoxin toxin can activate the apoptotic cycle and prevent cancer from progressing with planned death.
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