Volume 28, Issue 2 (4-2021)                   RJMS 2021, 28(2): 23-32 | Back to browse issues page

Research code: مقاله مستخرج از رساله دکتری است
Ethics code: IR.KMU.REC.1396.1562
Clinical trials code: مقاله مستخرج از رساله دکتری است

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Etemadi H R, Arshadi S, Banaeifar A, azarbayjani M. The effect of aerobic exercise and vitamin D supplementation on hepatic Apoptosis levels in rats exposed to oxygenated water. RJMS 2021; 28 (2) :23-32
URL: http://rjms.iums.ac.ir/article-1-6292-en.html
Islamic Azad University, South Tehran Branch, Tehran, Iran , Arshadi.sajad@yahoo.com
Abstract:   (1758 Views)
Background and Aims: Most chronic diseases are associated with an imbalance between the formation of reactive oxygen species (ROS) and antioxidant enzymes, which leads to deviation from the steady state. This process is called oxidative stress. One of the complications of oxidative stress is liver dysfunction. In this case, programmed cell death (apoptosis) appears to increase in liver cells and damage the tissue. Increased production of ROS without adequate antioxidant defense leads to oxidative stress and cell death and apoptosis, which is associated with various diseases and abnormalities. However, some studies have shown that exercise is associated with reduction in apoptosis. In this regard, Fernandez et al. (2012) showed that 10 weeks of regular swimming training in male rats increased anti-apoptotic proteins (Bcl-2 and Bcl-x) and decreased apoptotic proteins (BAD). also, Chen et al showed that 11 weeks of treadmill running increased Bcl-2 in rats with doxorubicin-induced chronic kidney disease, while caspase-8 levels decreased. On the other hand, studies have shown the benefits of vitamin D on liver health. Recent studies have reported an association between vitamin D3 deficiency and the incidence of liver disease. According to the research’s mentioned and in response to the ambiguities caused by the apoptotic or protective effects of exercise in various physiological situations, including exposure to oxidative stress, which has an undeniable effect on the body and vital organs, including the liver. Therefore, the present study intends to investigate the question of whether regular aerobic exercise and vitamin D supplementation effect on hepatic Apoptosis levels in rats exposed to oxygenated water?
Methods: This is an experimental study. This research was conducted in 2019 on Wistar rats in Kerman University of Medical Sciences, Kerman, Iran. 60 male Wistar rats weighed 220±20 g was randomly divided into ten groups. Then, The animals were exposed to oxidative stress (intraperitoneal injection of H2O2 3 times a week), vitamin D3 (intraperitoneal injection daily) and exercise on treadmill for 8 weeks (30 to 60 min, at speeds of 8 to 20 m/min) after the last training session liver tissue was extracted and Caspase-8 protein concentration measured by ELISA method.
Results: The results showed that Caspase-8 protein levels were higher in the H, 2H, HD and 2HD groups compared to the control group (p<0.01).Also, HE and HDE groups as well as 2HE and 2HDE groups showed lower levels of Caspase-8 protein in liver tissue compared to H and 2H groups, respectively (p<0.001).
Conclusion: Vitamin D can enhance the production of anti-inflammatory mediators and appears to regulate immune function. Zheng et al. (2017) reported that vitamin D receptor deficiency in human breast and prostate cancers significantly induces cell apoptosis by regulating the Wnt / β-catenin signaling pathway. The reasons for this discrepancy in the results can be traced to the dose of vitamin D consumed, which was higher in the studies than in the present study.
The findings of this study were consistent with the results of Fernandes et al. (2012) and Chen et al. Increased potential activity of antioxidant enzymes (not measured in the present study) may be practiced as one of the mechanisms for controlling apoptosis in animals. It is recommended that regular aerobic exercise be considered to reduce hepatic apoptosis. In the present study, there were limitations in the present study, including the study of animal specimens. Other limitations of this study include lack of measurement of other apoptosis-related factors.
the results showed that exposure of rats to H2O2 induces apoptosis in liver tissue. regular treadmill exercise was able to attenuate this effect to a large extent, but vitamin D3 supplementation did not.
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Type of Study: Research | Subject: Exercise Physiology

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