Volume 26, Issue 6 (9-2019)
RJMS 2019, 26(6): 33-43 |
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Barzegari A, Mirdar S, Ranaee M. Evaluation of immunohistochemical expression of Annexin A2 and histopathologic changes of lung tissue in rats exposed to nicotine nitrosamine ketone and Nigella sativa nanocapsules. RJMS 2019; 26 (6) :33-43
URL: http://rjms.iums.ac.ir/article-1-5534-en.html
URL: http://rjms.iums.ac.ir/article-1-5534-en.html
Department of physical education and sport, Payame Noor University, PO BOX 19395-3697, Tehran, IRAN , ali_barzegari@pnu.ac.ir
Abstract: (2935 Views)
Backgrounds: One of the powerful carcinogens in tobacco is nicotine nitrosamine ketone (NNK), which can activate annexin a2 and develop the irritation of the lung. Since the Nigella sativa plays a vital role in treating cancer, the aim of this research was to evaluation of annexin a2 expression and histopathologic changes of lung tissue in rats exposed to NNK and Nigella sativa nanocapsules.
Methods: In this experimental research, 45 Wistar male and female rats with average weight and standard deviation of 103.83 ± 23.48 grams were haphazardly separated into five groups including supplement, supplement + NNK, NNK, saline and control. NNK-induced groups received NNK subcutaneously one day per week at a rate of 12.5 mg/kg/bw. Supplemented groups similarly consumed Nigella sativa nanocapsules for 12 weeks at 12.5 μg / kg body weight. Annexin a2 expression in lung tissue were quantified by immunohistochemistry technique. One-way ANOVA and tukey’s multiple comparisons test were utilized for analyzing the data.
Results: Exposure to NNK significantly augmented annexin a2 expression compared to the saline group (P<0.001). On the other hand, a period of Nigella sativa nanocapsules consumption significantly decreased the annexin a2 expression in supplement + NNK group compared to the NNK group (P<0.001).
Conclusion: It seems that Nigella sativa nanocapsules consumption along with other therapeutic methods, can be useful in reducing the role of carcinogenic NNK and inflammation caused by cigarette smoke.
Methods: In this experimental research, 45 Wistar male and female rats with average weight and standard deviation of 103.83 ± 23.48 grams were haphazardly separated into five groups including supplement, supplement + NNK, NNK, saline and control. NNK-induced groups received NNK subcutaneously one day per week at a rate of 12.5 mg/kg/bw. Supplemented groups similarly consumed Nigella sativa nanocapsules for 12 weeks at 12.5 μg / kg body weight. Annexin a2 expression in lung tissue were quantified by immunohistochemistry technique. One-way ANOVA and tukey’s multiple comparisons test were utilized for analyzing the data.
Results: Exposure to NNK significantly augmented annexin a2 expression compared to the saline group (P<0.001). On the other hand, a period of Nigella sativa nanocapsules consumption significantly decreased the annexin a2 expression in supplement + NNK group compared to the NNK group (P<0.001).
Conclusion: It seems that Nigella sativa nanocapsules consumption along with other therapeutic methods, can be useful in reducing the role of carcinogenic NNK and inflammation caused by cigarette smoke.
Keywords: Nigella sativa nanocapsules, annexin A2, 4-((hydroxymethyl)nitrosamino)-1-(3-pyridyl)-1-butanone, Rats.
Type of Study: Research |
Subject:
Immunology
References
2. 1. Bade BC, Thomas DD, Scott JB, Silvestri GA. Increasing Physical Activity and Exercise in Lung Cancer Reviewing Safety, Benefits, and Application. J Thorac Oncol; 2015. 10: 861-871.
3. 2. Das A, Bortner JD, Aliagaa C, Cooper T, Stanley A, Stanley BA, et al. Proteomic profiling of hyperplasia/atypia and adenoma-induced by NNK in mouse lung identified multiple proteins as potential biomarkers for early detection. EuPA Open Proteomics; 2015. 9: 23–33.
4. 3. Koutsokera A, Kiagia M, Saif MW, Souliotis K, Syrigos KN. Nutrition Habits, Physical Activity, and Lung Cancer: An Authoritative Review. Clinical Lung Cancer; 2013. 14(4): 342-50.
5. 4. Indovina P, Marcelli E, Pentimalli F, Tanganelli P, Tarro G, Giordano A. Mass spectrometry-based proteomics: The road to lung cancer biomarker discovery. Mass Spectrom Rev; 2013. 32: 129-142.
6. 5. Peterson LA, Thomson NM, Crankshaw DL, Donaldson EE, Kenney PJ. Interactions between methylating and pyridyloxobutylating agents in A/J mouse lungs: implications for 4-
7. (methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced lung tumorigenesis. Cancer Res; 2001. 61: 5757-5763.
8. 6. Vernooy JHJ, Dentener MA, vanSuylen RJ, Buurman WA, Wouters EFM. Long-term intratracheal lipopolysaccharide exposure in mice results in chronic lung inflammation and persistent pathology. Am J Res Cell Mol Biol; 2002. 26: 152-159.
9. 7. Hecht SS, Stepanov I, Carmella SG. Exposure and Metabolic Activation Biomarkers of Carcinogenic Tobacco-Specific Nitrosamines. Acc Chem Res; 2016. 49(1): 106–114.
10. 8. Akopyan G, Bonavida B. Understanding tobacco smoke carcinogen NNK and lung tumorigenesis (Review). Int J Oncol; 2006. 29: 745-752.
11. 9. EL-Meghawry EL-Kenawy A. Apoptotic and anti-angiogenic activity of HypericumperforatumL on 4-(methylnitrosamino) - 1-(3-pyridyl)-1-butanone-induced lung cancer in rats. IJAR; 2014. 2(1):1057-1065.
12. 10. Conti-Fine BM, Navaneetham D, Lei S, Maus AD. Neuronal nicotinic receptors in non-neuronal cells: new mediators of tobacco toxicity? Eur J Pharmacol; 2000. 393: 279-294.
13. 11. Razani-Boroujerdi S, and Sopori ML. Early Manifestations of NNK-Induced Lung Cancer Role of Lung Immunity in Tumor Susceptibility. Am J Respir Cell Mol Biol; 2007. 36: 13-19.
14. 12. Barta P, Van Pelt C, Men T, Dickey BF, Lotan R, Moghaddam SJ. Enhancement of lung tumorigenesis in a Gprc5a knockout mouse by chronic extrinsic airway inflammation. Mol Cancer; 2012. 11:4
15. 13. Swisher JF, Khatri U, Feldman GM. Annexin A2 is a soluble mediator of macrophage activation. J Leukoc Biol; 2007. 82: 1174-1184.
16. 14. Rahimi H, Farajollahi M, Yazdanparast S, Azizi M, Khalaj V. Cellular Localization of AnnexinC3.1 and the Effect of its Overexpression on Growth and Protein Secretion in Aspergillus niger. Razi J Med Sci; 2009. 16: 121-129.
17. 15. Kirschnek S, Adams C, Gulbins E. Annexin II is a novel receptor for Pseudomonas aeruginosa. Biochem Biophys Res Commun; 2005. 327(3): 900-6.
18. 16. Cui JW, Wang YL. Expression and function of Annexin II in lung cancer tissue. Asian Pac J Trop Med; 2013. 6(2): 150-2.
19. 17. Bharadwaj A, Bydoun M, Holloway R, Waisman D. Annexin A2 Heterotetramer: Structure and Function Int. Int J Mol Sci; 2013. 14(3): 6259-6305.
20. 18. 22. Koutsokera A, Kiagia, M, Saif MW, Souliotis K, Syrigos, KN. Nutrition Habits, Physical Activity, and Lung Cancer: An Authoritative Review. Clinical Lung Cancer; 2013. 342-350.
21. 19. Rahmani AH, Alzohairy MA, Khan MA, Aly SM. Therapeutic Implications of Black Seed and Its Constituent Thymoquinone in the Prevention of Cancer through Inactivation and Activation of Molecular Pathways. Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine; 2014. 1-13.
22. 20. Paramasivam A, Raghunandhakumar S, Sambantham S, Anandana B, Rajiv R, Priyadharsini JV, et al. In vitro anticancer and antiangiogenic effects of thymoquinone in mouse neuroblastoma cells (Neuro-2a). Biomed Prevent Nut; 2012. 2: 283-6.
23. 21. Rashid Shyekh Ahmad M, Sanjarian F, Sabouni F. Toxicity effect of Nigella sativa and Nigella arvensis seed extract on gastric cancer cell lines (AGS) and brain tumor cells (AST). RJMS; 2016. 23(148): 72-79.
24. 22. Al-Sheddi ES, Farshori NN, Al-Oqail MM, Musarrat J, Al-Khedhairy AA, Siddiqui MA. Cytotoxicity of Nigella sativa seed oil and extract against human lung cancer cell line. Asian Pac J Cancer Prev. 2014. 15(2): 983-7.
25. 23. Herzog CR, Desai D, Amin S. Array CGH analysis reveal schromosomal aberrations in mouse lung adenocarcinomas induced by the human lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. BiochemBiophys Res Commun; 2006. 341: 856-863.
26. 24. Rafienia M, Orang F, Emamy SH. Preparation and characterization of polyurethane microspheres containing theophylline. J Bioact Compat Polymers; 2006. 21(4): 341-349.
27. 25. Olfert ED, Cross BM, McWilliam AA. Guide to the care and use of experimental animals. Canad Council Animal Care Ottawa; 1993. p. 1-193.
28. 26. Belinsky SA, Foley JF, White CM, Anderson MW, Maronpot RR. Dose-response relationship between O6-methylguanine formation in Clara cells and induction of pulmonary neoplasia in the rat by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. Cancer Res; 1990. 50(12): 3772-3780.
29. 27. Hansen HH. Lung Cancer (Advances in Basic and Clinical Research). Copenhagen, Springer Science+Business Media, Llc; 1995.
30. 28. Mirdar SH, Arabzadeh E, Arzani A, Ahmadi S, Neyestani F, baghbani M. Comparison of time periods and different patterns of tipper with black currant supplementation on weight variation and endurance performance of the growing male Wistar rats. J Sport Pec; 2013. 10(20): 115-128.
31. 29. Chen Q, Liang C1, Wang X, He J, Li Y, Liu Z. An albumin-based theranostic nano-agent for dual-modal imaging guided photo thermal therapy to inhibit lymphatic metastasis of cancer post-surgery. Biomaterials; 2014. 35(34): 9355-62.
32. 30. Keshavarzi M, Shakeri S, Kiani K. Preparation and in vitro investigation of antigastric cancer activities of carvacrol-loaded human serum albumin nanoparticles. IET Nanobiotechnol; 2015. 9(5):294-9.
33. 31. Verma SK, Rastogi S, Javed K, Akhtar M, Arora I, Samim M. Nanothymoquinone, a novel hepatotargeted delivery system for treating CCl4 mediated hepatotoxicity in rats. J Mats Chem; 2013. 1(23): 2956-2966.
34. 32. Jin L, Shen Q, Ding S, Jiang W, Jiang L, Zhu X. Immunohistochemical expression of Annexin A2 and S100A proteins in patients with bulky stage IB-IIA cervical cancer treated with neoadjuvant chemotherapy. Gynecol Oncol; 2012. 126(1):140-6.
35. 33. Wang CY, Chen CL, Tseng YL, Fang YT, Lin YS, Su WC, et al. Annexin A2 Silencing Induces G2 Arrest of Non-small Cell Lung Cancer Cells through p53-dependent and-independent Mechanisms. J Biol Chem; 2012. 287(39): 32512-24.
36. 34. Sharma MR, Koltowski L, Ownbey RT, Tuszynski GP, Sharma MC. Angiogenesis-associated protein annexin II in breast cancer. Selective expression in invasive breast cancer and contribution to tumor invasion and progression. Exp Mol Pathol; 2006. 81: 146-156.
37. 35. Banerjee AG, Liu J, Yuan Y, Gopalakrishnan VK, Johansson SL, Dinda AK, et al. Expression of biomarkers modulating prostate cancer angiogenesis: Differential expression of annexin II in prostate carcinomas from India and USA. Molecular Cancer; 2003. 2: 34.
38. 36. Lokman NA, Ween MP, Oehler MK, Ricciardelli C. The Role of Annexin A2 in Tumorigenesis and Cancer Progression. Cancer Microenviron; 2011; 4: 199-208.
39. 37. Chetcuti A, Margan SH, Russell P, Mann S, Millar DS, Clark SJ, et al. Loss of annexin II heavy and light chains in prostate cancer and its precursors. Cancer Res; 2001. 61: 6331-34.
40. 38. Tanaka T, Akatsuka S, Ozeki M, Shirase T, Hiai H, Toyokuni S. Redox regulation of annexin 2 and its implications for oxidative stress-induced renal carcinogenesis and metastasis. Oncogene; 2004. 23: 3980-3989.
41. 39. Barzegari A, Mirdar S. Investigating the Effect of One Period of Nigella Sativa Nano capsules consumption on the histopathological Structure and Vascular Endothelial Growth Factor Levels of the Lung Tissue of the Rats Exposed to Nicotine-Derived Nitrosamine Ketone. JRUMS; 2018. 17(5): 435-446.
42. 40. Ranjan AP, Mukerjee A, Gdowski A, Helson L, Bouchard A, Majeed M, Vishwanatha JK. Curcumin-ER Prolonged Subcutaneous Delivery for the Treatment of Non-Small Cell Lung Cancer. J Biomed Nanotechnol; 2016. 12: 1-10.
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