Razi Journal of Medical Sciences

ABSTRACT

 Attention to high prevalence of inflammatory disease of upper gastrointestinal system and determination of Helicobacter pylori, “a great importance etiology of gastric disorders”, this study was performed for rapid diagnostic evaluation of Helicobacter pylori. This observational & cross-sectional study was performed in 100 cases who suffered from upper gastrointestinal symptoms. By an internist, at least two gastric biopsies with endoscope (From antrum region) were taken, one of the biopsies was used in H-E staining (reference for gold standard method) and its results compared with Giemsa stain result for determination of validity if Giemsa staining. Other biopsy was used in one minute rapid urease for rapid diagnosis in patient with H.Pylori infection and in statistical analysis of the results sensitivity, specificity, positive and negative predictive Values (PPV,NPV) and positive and negative likelihood ratio (PLR,NLR) were used. In Giemsa staining sensitivity was reported to be 95.9% and specificity was 44.4% and for rapid urease test sensitivity was 69.9% and specificity 85.2% PPV of Giemsa staining and rapid urease test were 82.3% and 92.7% respectively, and NPVof were 80% and 51% respectively. PLR in Giemsa staining and rapid urease test were 1.7 and 4.7 respectively and NLR were 0.09 and 0.35 respectively. Giemsa staining has a high sensitivity and its negative results is Valuable. Rapid Urease test has a high specificity, and its positive results are valuable but its negative results must be controlled with a sutiable reference test.