Razi Journal of Medical Sciences

  Background: The goal of this study was to evaluate the effect of two probiotics (Lactobacillus casei and Lactobacillus paracasei) with a peribiotic (Raftilose) on the growth and liver enzyme (ALP, ALT and AST) in rat.

  Methods : In this laboratory experimental study forty eight male rats (175± 25 g) were randomly divided into four experimental groups each with triplicate. One group was selected as control and three groups were fed with probiotics (5×108 CFU/ml), peribiotic (5% of the diet) and a mixture of probiotics with peribiotic with the same concentrations, respectively. The animals were fed with probiotic and peribiotic for 4 weeks and trial was continued for two weeks later without receiving any additives. Sampling was scheduled at days of 0 and 30 for biometry and days of 30 and 45 for liver enzyme assay. Data were analyzed using one-way ANOVA by SPSS software.

  Results: Lactobacillus casei and L. paracasei with Raftilose statistically had no effect on the rat growth (p<0.05). Also, results indicated that liver enzyme activity could alter independently. The amount of ALT was higher in the animal that received probiotics (p=0.032) than the control group at the day of 30. The level of ALP and AST not showed any statistical differences (p<0.05) between the groups.

  Conclusions: It should be concluded that addition of Lactobacillus casei and Lactobacillus paracasei with Raftilose as a prebiotic did not affect the rat growth, but the level of ALT could increase with the probiotics. Further study should be done for obtain the best results on feeding with probiotic and prebiotic in rat.