Research code: IR.SSRC.REC.1402.047
Ethics code: IR.SSRC.REC.1402.047
Clinical trials code: IR.SSRC.REC.1402.047
1- department of physical education and sport science, SR.C, Islamic Azad University, Tehran, Iran.
2- Department of Physical Education and Sports Sciences,SR.C. Islamic Azad University, Tehran, Iran ,abednatanzi@iau.ir
3- Department of Physical Education and Sports Sciences,SR.C .Islamic Azad University, Tehran, Iran, Department of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2- Department of Physical Education and Sports Sciences,SR.C. Islamic Azad University, Tehran, Iran ,
3- Department of Physical Education and Sports Sciences,SR.C .Islamic Azad University, Tehran, Iran, Department of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract: (84 Views)
Melanoma cancer is the most severe subset of skin cancer, which has high invasiveness and rapid metastasis to other organs. Recently, the role of Exercise training in the prevention and treatment of cancer has received much attention. Pineapple also belongs to the bromolyase family and the bromoloid subfamily, whose anti-cancer properties have been suggested. Matrix metalloproteinases are a family of proteolytic enzymes that destroy the extracellular matrix and basement membrane, and in this sense, they are important in physiological and pathological processes. Previous reports showed that MMP-2 and MMP-9 are essentially expressed in malignant melanomas, and their expression is strongly related to melanoma atypia and dedifferentiation in melanocytic lesions, so the aim of this study is to study the changes in gene expression of metalloproteins MMP-2 and MMP-9 on liver tissue and tumor volume after aerobic exercise and consumption of pineapple extract in mice with melanoma cancer. This study was conducted on 32 heads of C57 mice in four groups including control, aerobic training, pineapple extract and aerobic training-pineapple. After tumor induction to mice, the program of aerobic training and pineapple extract at the dose of 300mg/kg by gavage was implemented for six weeks. The weight and tumor volume of mice were measured. After obtaining blood and tissue samples, gene expression of MMP-2 and MMP-9 metalloproteins in liver tissue was performed by RT-PCR method. Then the data were analyzed using one-way analysis of variance, two-factor analysis of variance and post hoc test, and the significance level of p≤0.05 was considered. The findings showed that compared to the control group, aerobic training and pineapple-training led to a significant decrease in tumor volume and a decrease in the gene expression of metalloproteins MMP-2 and MMP-9 in the liver tissue.
Type of Study: Research |
Subject:
Exercise Physiology
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