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Research code: LU. ECRA. 2018.16
Ethics code: LU. ECRA. 2018.16
Clinical trials code: LU. ECRA. 2018.16

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Azari N, Rahmati M, Mirnasouri R. The Effect of Endurance Training and Consumption of Hydroalcoholic Extract of Urtica Dioica on the Number of Gastrocnemius Muscle Nuclei in STZ-Induced Diabetic Rats. RJMS 2024; 31
URL: http://rjms.iums.ac.ir/article-1-8164-en.html
Lorestan University , rahmati.mas@lu.ac.ir
Abstract:   (244 Views)
Background and Aims: The most responsive tissue to insulin in the body, which plays a major role in maintaining systemic glucose homeostasis, is skeletal muscle. Therefore, changes in the health of skeletal muscles can affect the glucose homeostasis of the whole body. One of the organelles that changes in diseases is the cell nucleus. Diabetes is characterized by elevated blood glucose due to defects in insulin secretion, insulin action, or both. Studies have shown that the diabetic environment enhances protein degradation, causes skeletal muscle atrophy and loss of myonuclei. The aim of the present study was to investigate the effect of endurance training and consumption of hydroalcoholic extract of Urtica dioica on the number of Gastrocnemius muscle nuclei in STZ-induced diabetic rats.
Methods: In this experimental research, 60 male Wistar rats were randomly divided into five groups: healthy-control (H-C), diabetes-control (D-C), diabetes-exercise (D-Ex), diabetes-Urtica dioica (D-Ud), diabetes-Urtica dioica-exercise (D-Ud-Ex). After the induction of diabetes by STZ injection, the rats of the exercise group performed the endurance exercise protocol for 6 weeks (moderate intensity, 5 days/ week, 50-55% VO2max). Rats in supplemented groups received hydroalcoholic extract of Urtica dioica with doses of 50 mg/kg by gavage method. After completing the protocol and extracting the gastrocnemius muscle, the immunohistochemical method was used to count the myonuclei. Shapiro-Wilk test was used to ensure the normal distribution of variables. Also, the Brown-Forsythe test was used to check the homogeneity of the variance of the groups. Significance level was considered P<0.05 in all cases. One-way ANOVA statistical test and Graph Pad Prism software (version 9) were used for data analysis.
Results: Two weeks after STZ injection, body weight in diabetes-Urtica dioica (P=0.001), diabetes-exercise (P=0.034) and diabetes-exercise-Urtica dioica (P=0.025) groups compared to the healthy group. The reduction control and this reduction continued until the end of the sixth week. Also, six weeks of endurance exercise and consumption of Urtica dioica extract significantly reduced blood glucose in the diabetes-exercise (P=0.001), diabetes-Urtica dioica (P=0.001), and diabetes-Urtica dioica-exercise (P=0.001) groups, compared with diabetes-control group. In addition, a significant difference was observed between diabetes-Urtica dioica group with diabetes-Urtica dioica-exercise (P=0.001) and diabetes-exercise (P=0.002) groups. Also, diabetes decreased the number of myonuclei (P=0.0198); Consumption of Urtica dioica extract (P=0.075) and six weeks of endurance exercise (P<0.0001) each alone and in combination together (P<0.001) increased the number of myonuclei in diabetic groups.
Conclusion: The findings showed that diabetes decreased the number of myonuclei and increased blood glucose in diabetic rats. On the other hand, the consumption of Urtica dioica hydroalcoholic extract, endurance exercise and combined intervention of the two together increased the number of myonuclei and decreased blood glucose in diabetic rats. In short, the effects of the Urtica dioica plant in the treatment of diabetes and its complications can be attributed to its effective chemical compounds and the anti-oxidative and anti-inflammatory effects of this plant.
Key words: Endurance Exercise, Urtica Dioica, Nuclei, Gastrocnemius Muscle, Diabetes
Conflict of interest: None declared
Funding: This study did not have any funds

Type of Study: Research | Subject: Exercise Physiology

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