Research code: IR.UMZ.REC.097004
Ethics code: IR.UMZ.REC.097004
Clinical trials code: IR.UMZ.REC.097004
Faculty of Physical Education & Sport Sciences, University of Mazandaran, Babolsar, Ira , mousavi2753@gmail.com
Abstract: (218 Views)
Background: The aim of this study was to assessing the protective effect of Resistance Training and Vitamin D on the histopathological changes of spinal cord tissue in Female Rats with Experimental Autoimmune Encephalomyelitis.
Material and method: In this experimental study, 48 male Louise 4-week-old female rats were randomly divided into 8 groups. Resistance training protocol was performed for 6 weeks and 5 days a week. Starting a training program with a load equal to 50% of the body weight of the rat. Animals in the first two sessions performed 8 to 10 repetitions (climbing the ladder) at 2-minute breaks. The rats were immunized with the guinea pig's spit and complete adjuvant. The EAE model was induced at the end of the sixth week of exercise. Rats received 5 μg of body weight per kilogram of vitamin D3 in 150 μl of sesame oil every two days once for 2 weeks’ intraperitoneal injection. selected microscopic sections of spinal cord tissue, then stained with hematoxylin and eosin were studied with optical microscope.
Results: Histopathological findings of the spinal cord showed that healthy control, D healthy control and healthy D resistance group were spinal cord tissue completely normal. In the MS control group, in addition to severe myelin degradation, inflammation was observed. In the healthy resistance training and MS resistance groups was observed vascular Hyperemia. In the D MS control group was found inflammation and myelin degradation, and in the D MS resistance group, inflammation and myelin degradation were partially.
Conclusion: According to the results, it seems that resistance exercise with vitamin D consumption can prevent the destruction of myelin and inflammation of the spinal cord tissue.
Material and method: In this experimental study, 48 male Louise 4-week-old female rats were randomly divided into 8 groups. Resistance training protocol was performed for 6 weeks and 5 days a week. Starting a training program with a load equal to 50% of the body weight of the rat. Animals in the first two sessions performed 8 to 10 repetitions (climbing the ladder) at 2-minute breaks. The rats were immunized with the guinea pig's spit and complete adjuvant. The EAE model was induced at the end of the sixth week of exercise. Rats received 5 μg of body weight per kilogram of vitamin D3 in 150 μl of sesame oil every two days once for 2 weeks’ intraperitoneal injection. selected microscopic sections of spinal cord tissue, then stained with hematoxylin and eosin were studied with optical microscope.
Results: Histopathological findings of the spinal cord showed that healthy control, D healthy control and healthy D resistance group were spinal cord tissue completely normal. In the MS control group, in addition to severe myelin degradation, inflammation was observed. In the healthy resistance training and MS resistance groups was observed vascular Hyperemia. In the D MS control group was found inflammation and myelin degradation, and in the D MS resistance group, inflammation and myelin degradation were partially.
Conclusion: According to the results, it seems that resistance exercise with vitamin D consumption can prevent the destruction of myelin and inflammation of the spinal cord tissue.
Type of Study: Research |
Subject:
Exercise Physiology
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