Volume 29, Issue 11 (1-2023)
RJMS 2023, 29(11): 0-0 |
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Vahabzadeh G, Nourozi S, Namazi R, Najafizadeh P. The Effect of Quercetin on Saos2 Osteosarcoma Cell Line. RJMS 2023; 29 (11)
URL: http://rjms.iums.ac.ir/article-1-7275-en.html
URL: http://rjms.iums.ac.ir/article-1-7275-en.html
, gelareh.vahabzadeh@gmail.com
Abstract: (196 Views)
Background and Aims:
Osteosarcoma is a progressive non-hematopoietic malignant neoplasm caused by the transformation of primary cells of mesenchymal origin. This disease is very aggressive and the tumor formed is fixed, hard and irregular. Quercetin is a natural flavonol in the bioflavonoids group with few side effects, which has antioxidant, anti-inflammatory and anti-cancer activity. The aim of this study is to evaluate quercetin as an anticancer agent on soas2 cells.
Materials and Methods:
After 24 hours of culture of Saos2 cells in 96-well plates, different concentrations of quercetin were added to the wells for 72 hours. Cell viability and nitric oxide production were evaluated by MTT assay and the Griess method, respectively. The data were analyzed by one way ANOVA and P < 0.05 was considered statistically significant.
Results:
The results of this study showed that quercetin can decrease the percentage of cell viability compared to the control group. The best effective dose is 120 μM. Also, the data showed that quercetin in all concentrations was able to reduce the production of nitric oxide (NO) levels in Saos2 cells and the best effective concentration is 120 μM.
Conclusion:
In this study, it was found that quercetin was able to reduce the viability of Saos2 cells, and part of its effects could be mediated partially by a decrease in NO production.
Osteosarcoma is a progressive non-hematopoietic malignant neoplasm caused by the transformation of primary cells of mesenchymal origin. This disease is very aggressive and the tumor formed is fixed, hard and irregular. Quercetin is a natural flavonol in the bioflavonoids group with few side effects, which has antioxidant, anti-inflammatory and anti-cancer activity. The aim of this study is to evaluate quercetin as an anticancer agent on soas2 cells.
Materials and Methods:
After 24 hours of culture of Saos2 cells in 96-well plates, different concentrations of quercetin were added to the wells for 72 hours. Cell viability and nitric oxide production were evaluated by MTT assay and the Griess method, respectively. The data were analyzed by one way ANOVA and P < 0.05 was considered statistically significant.
Results:
The results of this study showed that quercetin can decrease the percentage of cell viability compared to the control group. The best effective dose is 120 μM. Also, the data showed that quercetin in all concentrations was able to reduce the production of nitric oxide (NO) levels in Saos2 cells and the best effective concentration is 120 μM.
Conclusion:
In this study, it was found that quercetin was able to reduce the viability of Saos2 cells, and part of its effects could be mediated partially by a decrease in NO production.
Type of Study: Research |
Subject:
Pharmacology
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