Volume 27, Issue 10 (12-2020)                   RJMS 2020, 27(10): 167-177 | Back to browse issues page

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Foladi F, Azarbayjani M A, Peeri M, Ghazaliyan F. The Effect of Purslane (Portulaca Oleracea) Seed Hydroalcoholic Extract and Aerobic Training on Mitochondrial Biogenesis Markers and DNA Damage in Liver Tissues in Rats Toxicated by Hydrogen Peroxide. RJMS 2020; 27 (10) :167-177
URL: http://rjms.iums.ac.ir/article-1-6031-en.html
Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran , m_azarbayjani@iauctb.ac.ir
Abstract:   (734 Views)
Background & Aims: Under normal conditions, the aerobic metabolism of the liver takes place with the constant production of pro-oxidants such as reactive oxygen species, which maintain the balance through their consumption at a similar rate by antioxidants. Slow imbalance in the ratio of prooxidants-antioxidants suggests the hypothesis of oxidative stress in body tissues. Oxidative stress is a condition during which the prooxidant-antioxidant balance (PAB) is disrupted and the redox state (oxidation-regeneration) leads to the disruption of this balance. During this process, free radicals are created on the surface of the cell membrane and cause damage to the cell membrane and the membrane of the organelles inside the cell, especially mitochondria. On the other hand, the abnormal increase in lipid peroxidation leads to damage to cell membranes and organelles. Malondialdehyde (MDA) is the final product of fatty acid oxidation, and measuring the level of malondialdehyde as an index of lipid peroxidation is of special clinical importance in determining the amount of free radicals. An increase in the tissue level of malondialdehyde is a sensitive and specific criterion related to lipid autooxidation. In the conditions of increasing or decreasing inhibition of free radicals, an imbalance in prooxidant expression is created, which is the basis and principle of the pathogenesis of acute and chronic diseases. Hepatic is mentioned. It has been found that regular physical exercises increase the antioxidant status in many tissues, including the liver. Some studies have shown the positive effects of physical exercise on improving the ratio of prooxidants and factors involved in mitochondrial biogenesis and DNA degradation. On the other hand, the evidence shows that in various physiological and pathological conditions, endogenous antioxidants cannot completely prevent oxidative damage. Purslane, with the scientific name Portulaca Oleracea, belongs to the genus Portulaca and is a widely used plant among medicinal plants, it is a strong source of omega-3 fatty acids, beta-carotene, ballotins, kaempferol, quercetin, apigenin, glutathione, noradrenaline, and dopamine. As mentioned, the imbalance between prooxidant and antioxidant factors will increase oxidative stress markers, which will ultimately affect the mitochondrial function of the cell. The beneficial effects of moderate-intensity aerobic exercise on preventing the progression of mitochondrial disorders have been shown, but the mechanisms involved are not well understood. Studies show that the increase in the amount of reactive oxygen species in both chronic and acute forms, under pathophysiological conditions, is essential for the development of liver diseases. Antioxidants that are effective against ROS can play a major role in limiting liver diseases and their clinical disorders such as diabetes, and fatty liver disease. Therefore, despite these problems and disorders, it is necessary to investigate the ratio of prooxidants and antioxidants and the balance between them, and gaining a correct view of this situation can help people who are prone to liver diseases and follow-up treatment with Antioxidant supplements are of great help. Considering the potential role of purslane seeds and regular physical exercises to deal with oxidative damage, this research aims to investigate the effect of purslane seeds and aerobic exercise on mitochondrial biogenesis markers and liver tissue DNA damage in female rats poisoned with hydrogen peroxide.
Methods: In an experimental trial, 54 male Wistar rats were randomly divided into 9 groups. All groups received 100 mg/kg body weight of hydrogen peroxide (H2O2) intraperitoneally for 14 days. Rats in the supplemented groups received purslane hydroalcoholic extract with doses of 50, 200, and 400 mg per day by gavage method. Aerobic exercise was performed on a treadmill at a speed of 23 meters per minute, 30 minutes per day, 5 days per week, and for eight weeks. The data were analyzed using a two-way analysis of variance and the Bonferroni post hoc test at a significance level of P<0.05.
Purslane seeds and the combined intervention of purslane seeds with aerobic training led to a significant increase in the levels of O-6-methylguanine-DNA-methyltransferase and oxidant-prooxidant balance (PAB), as well as a significant decrease in adenosine triphosphate (ATP levels ) and malondialdehyde (MDA) in the liver tissue of female rats poisoned with hydrogen peroxide (P<0.05). The interactive effect of aerobic exercise and purslane seeds was higher than supplementation and exercise alone.
Conclusion: According to the findings, the combined intervention of regular aerobic exercises and consumption of different doses of purslane supplement is a modulating factor in mitochondrial biogenesis and effective in reducing liver tissue DNA damage. In summary, the results of the present research showed that purslane seeds and the combined intervention of purslane seeds with aerobic exercise are modulating factors in mitochondrial biogenesis and are effective in reducing liver tissue DNA damage. Therefore, it is recommended to use the combined intervention of aerobic exercise and purslane seeds as a preventive method for liver damage. In the future, it is suggested to conduct a study on different protocols of aerobic exercise and purslane seeds on the structure and function of liver tissue. The results of the present study showed that aerobic exercise led to the improvement of PAB, MDA, ATP, and 6-methylguanine levels in the liver tissue of female rats poisoned with hydrogen peroxide. About the effect of exercise on mitochondrial biogenesis and DNA damage in liver tissue, there have been few studies that have expressed different mechanisms in the interpretation of the results. Several studies have tried to find effective mechanisms in improving mitochondrial biogenesis and DNA damage following exercise; The body has sufficient antioxidant reserves to deal with ROS production under physiological conditions, but when ROS production increases, as occurs during exercise, the imbalance between prooxidants and antioxidants favors prooxidants may occur and may lead to disruption of redox control and signaling or molecular damage.
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Type of Study: Research | Subject: Exercise Physiology

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