Volume 26, Issue 12 (2-2020)
RJMS 2020, 26(12): 103-110 |
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Bagheri-Hosseinabadi Z, Javani Jouni F, Vazini H, Elikaei A. Anti-cancer effects of hydatid cyst antigens on SK-BR-3 cancer cell lines. RJMS 2020; 26 (12) :103-110
URL: http://rjms.iums.ac.ir/article-1-5912-en.html
URL: http://rjms.iums.ac.ir/article-1-5912-en.html
Medical Parasitology, PhD. Nursing department, Basic sciences faculty, Hamedan Branch, Islamic Azad University, Hamedan, Iran. , hossein_vazini@yahoo.com
Abstract: (2635 Views)
Background: Cancer is a disease that starts from abnormal proliferation of cells in the body and occurs in different tissues of the host body. Cancer treatment is currently being treated with chemical drugs and radiation that can have long-term complications for the patient. The aim of this study was to investigate the anticancer effects of hydatid cyst antigens on SK-BR-3 cancer cell lines.
Methods: In this study, hydatid cysts were collected from slaughterhouses in the province of Isfahan and the fluid removed with Protoscolex. Crude antigens, cyst fluid antigens and excretory-secretory antigens were extracted. SK-BR-3 cell line was cultured in RPMI medium. Different antigen fractions were added to the cells in 96-well plates and after 48 hours of treatment, cell viability was assessed using MTT assay.
Results: All fractions had acceptable anti-cancer effects compared to the control group, which was statistically significant. The fraction of 60% of ammonium sulfate of the crude antigen had better anticancer effects than the 20% and 40% fraction, but in the liquid cyst antigen and the excretory-secretory antigen, the anticancer activity of the ammonium sulfate 40% fraction was higher than the other fractions.
Conclusion: The results of the present study showed that antigens isolated from human hydatid cysts have anticancer effects on SK-BR-3 cancer cell line and can be used in future studies as candidates for cancer prevention.
Methods: In this study, hydatid cysts were collected from slaughterhouses in the province of Isfahan and the fluid removed with Protoscolex. Crude antigens, cyst fluid antigens and excretory-secretory antigens were extracted. SK-BR-3 cell line was cultured in RPMI medium. Different antigen fractions were added to the cells in 96-well plates and after 48 hours of treatment, cell viability was assessed using MTT assay.
Results: All fractions had acceptable anti-cancer effects compared to the control group, which was statistically significant. The fraction of 60% of ammonium sulfate of the crude antigen had better anticancer effects than the 20% and 40% fraction, but in the liquid cyst antigen and the excretory-secretory antigen, the anticancer activity of the ammonium sulfate 40% fraction was higher than the other fractions.
Conclusion: The results of the present study showed that antigens isolated from human hydatid cysts have anticancer effects on SK-BR-3 cancer cell line and can be used in future studies as candidates for cancer prevention.
Type of Study: Research |
Subject:
parasitology
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