Volume 26, Issue 11 (1-2020)                   RJMS 2020, 26(11): 124-133 | Back to browse issues page

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ghorbanifar S, pornour M, sohrabi M. Evaluation of expression of miR-182-3p in blood circulation of patients with breast cancer. RJMS 2020; 26 (11) :124-133
URL: http://rjms.iums.ac.ir/article-1-5886-en.html
, ma.pornour@gmail.com
Abstract:   (2710 Views)
Background: Breast cancer is the most common cancer among women, especially in Asian women. miRNAs are a class of non-coding RNAs that play an important role in controlling gene expression at the post-transcriptional level. miR-182 is a tumor inducer in breast cancer and its expression in cancer tissue is increased. This study was performed to investigate the changes in miR-182-3p expression in blood plasma of breast cancer patients and its diagnostic value as a biomarker.
Methods: This study is a case-control study. Blood samples were collected from the 30 Patient with breast cancer and the 30 healthy persons (25 to 65 years) as controls. After plasma isolation, RNA was extracted and cDNA was synthesized. Blood plasma samples of the healthy individuals and the patients with breast cancer were measured by the Real Time PCR technique. The expression changes of miR-182-3p between plasma samples were evaluated by T-test.
Results: The expression level of miR-182-3p in the blood plasma of patients with breast cancer was significantly higher than that of healthy individuals (fold change = 606/8). Also, there was a significant relationship between miR-182-3p expression and early stage disease and stage I, II.
Conclusion: The results of this study showed that miR-182-3p has a significant increase in the blood plasma of people with breast cancer like cancer tissue. Therefore, it is likely to have the potential to be used as a diagnostic marker in the early and safe diagnosis of breast cancer.
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Type of Study: Research | Subject: Genetic

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