Volume 26, Issue 6 (9-2019)
RJMS 2019, 26(6): 95-104 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Karimi M, Eizadi M. The effect of interval training on FOXO1 expression in pancreas tissue of diabetes rats with high fat diet and STZ. RJMS 2019; 26 (6) :95-104
URL: http://rjms.iums.ac.ir/article-1-5834-en.html
URL: http://rjms.iums.ac.ir/article-1-5834-en.html
Qom University of Technology, Qom, Iran , karimi.m@qut.ac.ir
Abstract: (3755 Views)
Background: The synthesis and secretion of insulin from the pancreas and insulin function in target tissues are one of the most important causes of the prevalence or severity of type 2 diabetes. In present study, the aim is to determine the effect of high intensity interval training (HIIT) training on FOXO1 expression in pancreas tissue of type 2 diabetes rats (T2D).
Methods: T2D induced by high fat diet and intraperitoneal STZ injection in 14 male Wistar rats and randomly divided into test (n = 7) or control (n = 7) groups. The test subjects were completed 6 weeks HIIT (5 days/weekly) in the form of running on a treadmill and control remained with no exercise in this period. FOXO1 expression in pancreas tissue, fasting glucose and serum insulin were determined after training program of 2 groups. Independent t-test was used at a significant level of less than 5% for comparing variables between groups.
Results: HIIT resulted in significant increase in FOXO1 relative expression in pancreas tissue (p= 0.017). Compared to control group, fasting insulin decreased by HIIT (p= 0.007). Fasting glucose was also decreased in exercise than control group (p<0.0001).
Conclusion: Based on this data, improve serum insulin in test rats could be attributed to increased FOXO1 expression in pancreas tissue by HIIT. However, more studies are needed to clarify the mechanisms underling the effects of exercise on the synthesis and secretion of insulin.
Methods: T2D induced by high fat diet and intraperitoneal STZ injection in 14 male Wistar rats and randomly divided into test (n = 7) or control (n = 7) groups. The test subjects were completed 6 weeks HIIT (5 days/weekly) in the form of running on a treadmill and control remained with no exercise in this period. FOXO1 expression in pancreas tissue, fasting glucose and serum insulin were determined after training program of 2 groups. Independent t-test was used at a significant level of less than 5% for comparing variables between groups.
Results: HIIT resulted in significant increase in FOXO1 relative expression in pancreas tissue (p= 0.017). Compared to control group, fasting insulin decreased by HIIT (p= 0.007). Fasting glucose was also decreased in exercise than control group (p<0.0001).
Conclusion: Based on this data, improve serum insulin in test rats could be attributed to increased FOXO1 expression in pancreas tissue by HIIT. However, more studies are needed to clarify the mechanisms underling the effects of exercise on the synthesis and secretion of insulin.
Type of Study: Research |
Subject:
Exercise Physiology
References
1. 1. Chailurkit LO, Chanprasertyothin S, Jongjaroenprasert W, Ongphiphadhanakul B. Differences in insulin sensitivity, pancreatic beta cell function and circulating adiponectin across glucose tolerance status in Thai obese and non-obese women. Endocrine; 2008. 33(1):84-9.
2. 2. Retnakaran R, Hanley AJ, Raif N, Hirning CR, Connelly PW, Sermer M, et al. Adiponectin and beta cell dysfunction in gestational diabetes: pathophysiological implications. Diabetologia; 2005. 48(5):993-1001.
3. 3. Kim DJ, Lee MS, Kim KW, Lee MK. Insulin secretory dysfunction and insulin resistance in the pathogenesis of Korean type 2 diabetes mellitus. Metabolism; 2001. 50(5):590-3.
4. 4. Matsumoto K, Miyake S, Yano M, Ueki Y, Yamaguchi Y, Akazawa S, et al. Glucose tolerance, insulin secretion, and insulin sensitivity in nonobese and obese Japanese subjects. Diabet Care; 1997. 20(10):1562-8.
5. 5. Levy J, Atkinson AB, Bell PM, McCance DR, Hadden DR. Beta-cell deterioration determines the onset and rate of progression of secondary dietary failure in type 2 diabetes mellitus: the 10-year followup of the Belfast Diet Study. Diabet Med;
6. 1998 Apr. 15(4):290-6.
7. 6. Da Silva Xavier G, Qian Q, Cullen PJ, Rutter GA. Distinct roles for insulin and insulin-like growth factor-1 receptors in pancreatic beta-cell glucose sensing revealed by RNA silencing. Biochem J; 2004. 377(Pt 1): 149–158.
8. 7. Ogino J, Sakurai K, Yoshiwara K, Suzuki Y, Ishizuka N, Seki N, et al. Insulin resistance and increased pancreatic beta-cell proliferation in mice expressing a mutant insulin receptor (P1195L). J Endocrinol; 2006. 190(3): 739–747.
9. 8. MacDonald MJ, Fahien LA, Brown LJ, Hasan NM, Buss JD, Kendrick MA. Perspective: emerging evidence for signaling roles of mitochondrial anaplerotic products in insulin secretion. Am J Physiol Endocrinol Metabol; 2005 Jan. 288(1): 1-15.
10. 9. Ruchat SM, Rankinen T, Weisnagel SJ, Rice T, Rao DC, Bergman RN, et al. Improvements in glucose homeostasis in response to regular exercise are influenced by PPARG Pro12Ala variant: results from the HERITAGE Family Study. Diabetologia; 2010 Apr. 53(4):679-89.
11. 10. Ramazani Rad M, Hajirasouli M, Eizadi M. The Effect of 12 weeks of aerobic training on glp-1 receptor expression in pancreatic tissue and glycemic control in type 2 diabetic rats. Qom Uni Med Sci J; 2017. 11(6):36-45.
12. 11. Rashidi M, Soori R, Choobineh S, Ravasi AA, Baesi K. The Effect of an Aerobic Exercise on MTNR1B Gene Expression, Insulin and Glucose Levels in Pancreas of Induced Diabetic Rat with Streptozotocin-Nicotinamide. J Know Health; 2016. 11(3): 40-48.
13. 12. Poitout V, Olson LK, Robertson RP. Chronic exposure of betaTC-6 cells to supraphysiologic concentrations of glucose decreases binding of the RIPE3b1 insulin gene transcription activator. J Clin Invest; 1996 Feb 15. 97(4):1041-6.
14. 13. Accili D, Arden KC. FoxOs at the crossroads of cellular me- tabolism, differentiation, and transformation. Cell; 2004 May 14. 117(4):421-6.
15. 14. Kitamura YI, Kitamura T, Kruse JP, Raum JC, Stein R, Gu W, Accili D. FoxO1 protects against pancreatic beta cell failure through NeuroD and MafA induction. Cell Metabol; 2005 Sep.2(3):153-63.
16. 15. Almasi A, Behboudi tabrizi L, Izadi M. Investigation effect of 12-week High-Intensity Interval Training FOXO1 Gene Expression of Subcutaneous Adipose Tissue and Insulin Resistance in Type 2 Diabetic Rats. J Neyshabur Uni Med Sci; 2018. 6(2):12-20.
17. 16. Azad M, Khaledi N, Hedayati M. Effect of acute and chronic eccentric exercise on FOXO1 mRNA expression as fiber type transition factor in rat skeletal muscles. Gene; 2016 Jun 15. 584(2):180-4.
18. 17. Eizadi M, Ravasi AA, Soory R, Baesi K, Choobineh S. The Effect of Three Months of Resistance Training on TCF7L2 Expression in Pancreas Tissues of Type 2 Diabetic Rats. Avicenna J Med Biochem; 2016 June. 4(1):e34014.
19. 18. Soori R, Rashidi M, Choobineh S, Ravasi AA, Baesi K, Rashidy-Pour A. Effects of 12 weeks resistant training on MTNR1B gene expression in the pancreas and glucose and insulin levels in type 2 diabetic rats. Koomesh; 2017. 19(1):46-55.
20. 19. Farajtabar Behrestaq S, Shakeri N, Ghazalian F, Nikbakht H. The Effect of 12 Weeks Aerobic Training on the Mafa Gene Expression of Pancreas in the Male Wistar Rats Type 2 Diabetes. IJDO; 2018. 10(2):73-79.
21. 20. Sun YP, Lu NC, Parmley WW, Hollenbeck CB. Effect of cholesterol diets on vascular function and Atherogenesis in rabbits. Proc Soc Exp Bio Med; 2000.224(3): 166-71.
22. 21. Kalhor H, Peeri M, Matin Homaee H, Izadi M. The Effect of 6 Weeks Resistance Training and HITT on GLP-1 Gene Expression of Diabetic Rats. Iran JDiabet Obes; 2018. 10(1): 42-9.
23. 22. Coughlin CC, Finck BN, Eagon JC, Halpin VJ, Magkos F, Mohammed BS, et al. Effect of marked weight loss on adiponectin gene expression and plasma concentrations. Obesity (Silver Spring); 2007 Mar. 15(3):640-5.
24. 23. Kadoglou NP, Iliadis F, Angelopoulou N, Perrea D, Ampatzidis G, Liapis CD, et al. The anti-inflammatory effects of exercise training in patients with type 2 diabetes mellitus. Eur J Cardiovasc Prev Rehabil; 2007 Dec. 14(6):837-43.
25. 24. Bai Y, Zhang J, Jiang S, Sun J, Zheng C, Wang K, et al. Effects of the body fat mass and blood sugar and plasma resistin to slim exercise prescription for overweight and obesity students. Wei Sheng Yan Jiu; 2013 Jul. 42(4):538-42, 549.
26. 25. Jorge ML, de Oliveira VN, Resende NM, Paraiso LF, Calixto A, Diniz AL, et al. The effects of aerobic, resistance, and combined exercise on metabolic control, inflammatory markers, adipocytokines, and muscle insulin signaling in patients with type 2 diabetes mellitus. Metabolism; 2011 Sep. 60(9):1244-52.
27. 26. Ligtenberg PC, Hoekstra JB, Bol E, Zonderland ML, Erkelens DW. Effects of physical training on metabolic control in elderly type 2 diabetes mellitus patients. Clin Sci (Lond); 1997 Aug. 93(2):127-35.
28. 27. Oliveira CAM, Paiva MF, Mota CAS, Ribeiro C, Leme JACA, Luciano E, et al. Exercise at anaerobic threshold intensity and insulin secretion by isolated pancreatic islets of rats. Islets; 2010 Jul-Aug. 2(4):240-6.
29. 28. Huang HH, Farmer K, Windscheffel J, Yost K, Power M, Wright DE, et al. Exercise increases insu- lin content and basal secretion in pancreatic islets in type 1 diabetic mice. Exp Diabetes Res; 2011. 2011:481427.
30. 29. Fluckey JD, Kraemer WJ, Farrell PA. Pancreatic islet insulin secretion is increased after resistance exercise in rats. J Appl Physiol; 1995 Oct. 79(4):1100-5.
31. 30. Withers DJ, Gutierrez JS, Towery H, Burks DJ, Ren JM, Previs S, et al. Disruption of IRS-2 causes type 2 diabetes in mice. Nature; 1998. 391(6670): 900–904.
32. 31. Okamoto H, Nakae J, Kitamura T, Park BC, Dragatsis I, Accili D. Transgenic rescue of insulin receptor-deficient mice. J Clin Invest; 2004 Jul. 114(2):214-23.
33. 32. Buteau J, Accili D. Regulation of pancreatic b -cell f unction by the f orkhead protein FoxO1. Diabet Obes Metabol; 2007. 9 (Supp l. 2):140–146.
34. 33. Kitamura T, Nakae J, Kitamura Y, Kido Y, Biggs WH, Wright CV, et al. The forkhead transcription factor Foxo1 links insulin signaling to Pdx1 regulation of pancreatic beta cell growth. J Clin Invest; 2002 Dec. 110(12):1839-47.
35. 34. Buteau J, Shlien A, Foisy S, Accili D. Metabolic diapause in pancreatic beta-cells expressing a gain-of- function mutant of the forkhead protein Foxo1. J Biol Chem; 2007 Jan 5. 282(1):287-93.
36. 35. Toussaint O, Royer V, Salmon M, Remacle J. Stress- induced premature senescence and tissue ageing. Biochem Pharmacol; 2002 Sep. 64(5-6):1007-9.
37. 36. Chen Z, Meng C, Liu J, Zhang J, Kou Y, Zhang L, Wang Z. Effects of gastric bypass on FoxO1 expression in the liver and pancreas of diabetic rats. Endocr Res; 2016. 41(1):57-63.
38. 37. Zhang T, Kim DH, Xiao X, Lee S, Gong Z, Muzumdar R, et al. FoxO1 Plays an Important Role in Regulating β-Cell Compensation for Insulin Resistance in Male Mice. Endocrinology; 2016 Mar. 157(3):1055-70.
39. 38. Slopack D, Roudier E, Liu ST, Nwadozi E, Birot O, Haas TL. Forkhead BoxO transcription factors restrain exercise-induced angiogenesis. J Physiol; 2014 Sep 15. 592(18):4069-82.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
