Volume 26, Issue 7 (10-2019)                   RJMS 2019, 26(7): 131-140 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

jabbari E, Gholami M, Nikbakht H, Shakeri N, Ghazaliyan F. Effect of aerobic training and L-carnitine supplementation on some apoptotic factors in diabetic rat liver. RJMS 2019; 26 (7) :131-140
URL: http://rjms.iums.ac.ir/article-1-5798-en.html
Assistant Professor, Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran , m.gholami@srbiau.ac.ir
Abstract:   (2805 Views)
Background: The use of non-enzymatic antioxidants and proper exercise can have a positive effect on decreasing apoptotic side effect. The purpose of this study was to investigate the effect of aerobic training and consumption of l-carnitine on some of the apoptosis factors in the liver of diabetic rats.
Methods: In this experimental study, 45 male Wistar rats (200-300 gr) were randomly divided into six groups: 1) sham group, 2) healthy control group 3) diabetic control group, 4) diabetic group receiving l-carnitine, 5) diabetic group of aerobic training, 6) diabetic group of aerobic training and receiving l-carnitine. Rats were diabetic with nicotinic amide and a single dose of STZ of 55 mg / kg body weight. Rats in the L-carnitine group received daily 100 mg of l-carnitine orally. The aerobic exercise protocol done on the treadmill.
Results: Combined intervention of aerobic exercise and supplementation of l-carnitine had a significant effect on Bcl2 factor in liver tissue in diabetic rats. However, six weeks of aerobic exercise and supplementation alone do not have a significant effect, six weeks of aerobic training and combined intervention had significant effect on BAX factor in liver tissue in diabetic rats but supplementation of l-carnitine alone had no significant effect.
Conclusion: L-carnitine supplementation intake with regular exercise can have beneficial effects on apoptotic improvement in rats with type 2 diabetes
Full-Text [PDF 858 kb]   (1376 Downloads)    
Type of Study: Research | Subject: Physiology

1. References
2. 1. Jaffrey M. The effect of an aerobic training course with oral intake of cranberry on serum IL-17, IL-18, and cortisol levels in streptozotocin induced diabetic female rats. Master’s thesis, Islamic Azad University, Karaj branch. 2015. 56.
3. 2. Mohajeri D, Mousavi Gh, Kaffashi Elahi R, Neshat Gharamaleki M. Study on protective effect of Naringenin (Citrus flavonone) on incipient diabetic hepatopathy in alloxan-induced diabetic rats. Veter Clin Pathol; 2016. 10(1): 39-53.
4. 3. Hano T. Pathohistological study on the liver cirrhosis in diabetes mellitus. Kobe J Med Sci; 1968. 14:87-106.
5. 4. Doustar, Y. Mohajeri, D. Rezaei, A. Effects of grape seed extract on heart cells apoptosis in streptozotocin induced diabetic rats. Med sci j Islam azad uni; 2012. 21(3): 168-74.
6. 5. Frustaci A, Kajstura J, Chimenti C, Jakoniuk I, Leri A, Maseri A. Myocardial cell death in human diabetes. Circ Res; 2000. 87: 1123-32.
7. 6. Jaeschke H. Reactive oxygen and mechanisms of inflammatory liver injury. J.Gastroen. Hepatol; 2000. 15: 718–24.
8. 7. Mirdar Harijani Sh, Musavi N, Hamidian Gh. Effect of endurance swimming training during pregnancy on histology and apoptotic index of rats' liver. ISMJ; 2015. 18(1): 54-63.
9. 8. Lee B.J, Lin J.S, Lin Y.C, Lin P.T. Effects of L-carnitine supplementation on oxidative stress and antioxidant enzymes activities in patients with coronary artery disease: a randomized, placebo-controlled trial. Nut J; 2014. 13:79.
10. 9. Flanagan JL, Simmons PA, Vehige J, Willcox MD, Garrett Q. (). Role of carnitine in disease. Nutr Metab; 2010. 16. 7:30.
11. 10. Shokrzadeh M, Ahangar N, Zargari M, et al. Investigating the protective effect of L-Carnitine on the Malone De Aldehid level on lipid peroxidation of cell membrane in Diazinon-induced Rats. Med J Mazandaran Uni; 2012. 22(97): 198-206.
12. 11. Tousson E, Hafez E, Zaki S, Gad A. The cardioprotective effects of L-carnitine on rat cardiac injury, apoptosis, and oxidative stress caused by amethopterin. Environ Sci Pollut Res; 2016:1-9.
13. 12. Mansour H. (). Protective role of carnitine ester against radiation-induced oxidative stress in rats. Pharmacol Res; 2006. 54: 165-171.
14. 13. Bodea F, Bocea A, Decea N. L-carnitine decreases oxidative stress induced by experimental hypobaric hypoxia. Pediat Endocrinol Diabetes Metab; 2010. 16(2): 78-81.
15. 14. Kim HJ, Park JY, Lyul Oh S, Kim YA, So B, Seong JK. Effect of treadmill exercise on interleukin-15 expression and glucose tolerance in Zucker diabetic fatty rats. Diabetes Metab J; 2013. 37: 358-364.
16. 15. E Nico ETM, de Oliveira PR, de Souza LP, Pereira FDC, Delbin MA, Zanesco A. (). The action of aminoguanidine on the liver of trained diabetic rats. J Diabetes Metab Disord; 2013. 12(40): 1-9.
17. 16. Rodrigues B, Figueroa D, Mostarda C, Heeren M, Irigoyen M, Kátia D. Maximal exercise test is a useful method for physical capacity andoxygen consumption determination in streptozotocin-diabetic rats.Cardiovasc Dialectol; 2007. 6(38): 125-131.
18. 17. Falah, S. Kordi, M.R. Ahmadizadeh, S. et al. Effect of 8 weeks of Endurance Training on rest levels and responces of Visfanttin and Insulin Resistance index to acute endurance exercise in diabetic Rats. Sport Physiol Manage Invest; 2011. 8: 83-93.
20. 18. Jafari A, Pourrazi H, Nikookheslat S, Baradaran B. (). Effect of Exercise Training on Bcl-2 and Bax Gene Expression in the Rat Heart. Gene Cell Tissue; 2015.2(4): e32833.
21. 19. Ahmadiasl N, Ghadiri Soufi F, Alipour M, Bonyadi M, Sheikhzadeh F, Vatankhah A, Salehi I, Mesgari M. Effects of age increment and 36-week exercise training on antioxidant enzymes and apoptosis in rat heart tissue. Jour Sports Sci Med; 2007. 6:243-249.
22. 20. Sun Y, Cui D, Zhang Z, Zhang T, Shi J, Jin H, et al. Attenuated Oxidative Stress following Acute Exhaustive Swimming Exercise Was Accompanied with Modified Gene Expression Profiles of Apoptosis in the Skeletal Muscle of Mice. Oxid Med Cell Longev; 2016. 8381242.
23. 21. Mejías-Peña Y, Brisamar Estébanez, Paula Rodriguez-Miguelez, Rodrigo Fernandez-Gonzalo, Mar Almar, José A. de Paz, Javier González-Gallego, and María J. Cuevas. Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects. Aging; 2017. 9(2): 408–418.
24. 22. Santana ED, Serra AJ, Silva Junior JA. Aerobic exercise training induces an anti-apoptotic milieu in myocardial tiss. Motriz Rev Educ Fis; 2014. 20(2): 233-238.
25. 23. Marsh SA, Laursen PB, Pat BK, Gobe GC, Coombes JS. Bcl-2 in endothelial cells is increased by vitamin E and α-lipoic acid supplementation but not exercise training. J Mole Cell Cardiol; 2005. 38(3):445-51.
26. 24. Seo H, Chun-Hyung Park, Seokrip Choi, Woocheol Kim, Byung-Duk Jeon, and Seungpil Ryu Effects of voluntary exercise on apoptosis and cortisol after chronic restraint stress in mice. J Exerc Nutrition Biochem; 2016. 20(3): 16–23.
27. 25. Childs AC, Phaneuf SL, Dirks AJ, Phillips T, Leeuwenburgh C. Doxorubicin treatment in vivo causes cytochrome c release and cardiomyocyte apoptosis, as well as increased mitochondrial efficiency, superoxide dismutase activity, and Bcl-2: Bax ratio. Can Res; 2002. 62:4592-98.
28. 26. Mooren FC,Blöming D, Lechtermann A, Lerch MM,Völker K. Lymphocyte apoptosis after exhaustive and moderate exercise. Jour Appl Physiol; 2002. 93(1):147-53.
29. 27. Skommer J, Wlodkowic D, Deptala A. Larger than life: mitochondria and the Bcl-2 family. Leuk Res; 2007. 31:277-86.
30. 28. Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol; 2007. 35:495-516.
31. 29. Maulik N, Sasaki H, Addya S, Das DK. Regulation of cardiomyocyte apoptosis by redox-sensitive transcription factors. FEBS Lett; 2000. 485:7-12.
32. 30. Chen KC, Peng CC, Hsieh CL, Peng RY. Exercise ameliorates renal cell apoptosis in chronic kidney disease by intervening in the intrinsic and the extrinsic apoptotic pathways in a rat model. Evid-Based Complem Altern Med; 2013: 1-13
33. 31. Kwak HB, Song W, Lawler JM. Exercise training attenuates age-induced elevation in Bax/Bcl-2 ratio, apoptosis, and remodeling in the rat heart. FASEB J; 2006. 20(6): 791-3.
34. 32. Marin-Garcia J, Goldenthal MJ. Mitochondrial centrality in heart failure. Heart Fail Rev; 2008. 13: 137–150
35. 33. French JP, Hamilton KL, Quindry JC, Lee Y, Upchurch PA, Powers SK. Exercise-induced protection against myocardial apoptosis and necrosis: MnSOD, calcium-handling proteins, and calpain. FASEB J; 2008. 22: 2862–2871.
36. 34. Habibi P, Alihemmati A, NourAzar A, Yousefi H, Mortazavi S, Ahmadiasl N. Expression of the Mir-133 and Bcl-2 could be affected by swimming training in the heart of ovariectomized rats. Iran J Basic Med Sci;2016. 19(4):381.
37. 35. Cai MX, Shi XC, Chen T, Tan ZN, Lin QQ, Du SJ, et al. Exercise training activates neuregulin 1/ErbB signaling and promotes cardiac repair in a rat myocardial infarction model. Life Sci; 2016. 149:1-9.
38. 36. Lajoie C, Calderone A, Béliveau L. Exercise training enhanced the expression of myocardial proteins related to cell protection in spontaneously hypertensive rats. Pflügers Archiv; 2004. 449(1):26-32.
39. 37. Delchev S, Georgieva K, Koeva Y, Atanassova P. Bcl-2/Bax ratio, mitochondrial membranes and aerobic enzyme activity in cardiomyocytes of rats after submaximal training. Folia Mmed; 2005. 48(2):50-6.
40. 38. Siu PM, Bryner RW, Martyn JK, Alway SE. Apoptotic adaptations from exercise training in skeletal and cardiac muscles. FASEB J; 2004. 18(10):1150-2.
41. 39. Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP. Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol; 2011. 300:118-124.
42. 40. Cha, Y. S. (). Effects of L-carnitine on obesity, diabetes, and as an ergogenic aid. Asia Pac J Clin Nutr; 2008. 17(1): 306-308.
43. 41. Song W, Lu X, Feng Q. Tumor necrosis factor-α induces apoptosis via inducible nitric oxide synthase in neonatal mouse cardiomyocytes. Cardiovasc Res; 2000. 45:595-602.
44. 42. Davoodi M, Moosavi H, Nikbakht M. The effect of eight weeks selected aerobic exercise on liver parenchyma and liver enzymes (AST, ALT) of fat liver patients. Shahrekord Univ Med Sci J; 2012. 14(1):84-90.
45. 43. Bagci E, Vodovotz Y, Billiar T, Ermentrout G, Bahar I. Bistability in apoptosis: roles of bax, bcl-2, and mitochondrial permeability transition pores. Biophysic J; 2006. 90:1546-59.

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Razi Journal of Medical Sciences

Designed & Developed by : Yektaweb