Volume 26, Issue 6 (9-2019)
RJMS 2019, 26(6): 105-117 |
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Dousti B, Nabipour F, Hajiamraei A. Green Synthesis of Silver Nanoparticle Using Aqueous Extract of Fumaria Parviflora and Study of its Antibacterial and Antioxidant Properties. RJMS 2019; 26 (6) :105-117
URL: http://rjms.iums.ac.ir/article-1-5688-en.html
URL: http://rjms.iums.ac.ir/article-1-5688-en.html
Khorramabad Branch, Islamic Azad University, Khorramabad, Iran , dousti@khoiau.ac.ir
Abstract: (5149 Views)
Background: The bio-production method has been respected due to the lack of energy consumption and environmental compatibility. In this study, the green synthesis of silver nanoparticles was investigated from the extract of Fumaria Parviflora and its antioxidant and anti-bacterial properties compared to two common antibiotics.
Methods: First, aqueous extract of Fumaria Parviflorawas prepared and admixed with AgNo3solution at a concentration of 1 mM. After changing the color of extract, the reaction product was examined by spectrophotometric, X-ray diffraction and scanning electron microscopy. Finally, antioxidant properties and antibacterial properties of synthesized nanoparticles were investigated using DPPH method and disk diffusion method, respectively. Also, the MIC (minimum inhibitory concentration of growth and MBC (minimum bactericidal concentration) were determined by two methods of microdilution and dilution in the tube.
Results: The synthesized silver nanoparticles showed the most absorbance at 430 nm and had a spherical shape with an average size of 10-50 nm. The synthesized nanoparticles have antioxidant properties with IC50 of 21 μg / ml.The results of the antibacterial studies showed that the synthesized silver nanoparticles had more antibacterial activity against the gram-positive bacteria of Staphylococcus aureus than the gram-negative bacteria of Escherichia coli.
Conclusion: The extract of Fumaria Parviflora has the ability of reducing Ag+ ion to silver nanoparticles. Also, synthesized silver nanoparticles have good antioxidant and antibacterial activities.
Methods: First, aqueous extract of Fumaria Parviflorawas prepared and admixed with AgNo3solution at a concentration of 1 mM. After changing the color of extract, the reaction product was examined by spectrophotometric, X-ray diffraction and scanning electron microscopy. Finally, antioxidant properties and antibacterial properties of synthesized nanoparticles were investigated using DPPH method and disk diffusion method, respectively. Also, the MIC (minimum inhibitory concentration of growth and MBC (minimum bactericidal concentration) were determined by two methods of microdilution and dilution in the tube.
Results: The synthesized silver nanoparticles showed the most absorbance at 430 nm and had a spherical shape with an average size of 10-50 nm. The synthesized nanoparticles have antioxidant properties with IC50 of 21 μg / ml.The results of the antibacterial studies showed that the synthesized silver nanoparticles had more antibacterial activity against the gram-positive bacteria of Staphylococcus aureus than the gram-negative bacteria of Escherichia coli.
Conclusion: The extract of Fumaria Parviflora has the ability of reducing Ag+ ion to silver nanoparticles. Also, synthesized silver nanoparticles have good antioxidant and antibacterial activities.
Type of Study: Research |
Subject:
Microbiology
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