Volume 26, Issue 9 (12-2019)
RJMS 2019, 26(9): 39-46 |
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Alizadeh Sarvandani S, Amini K, Saffarian P. Evaluation of antimicrobial activity of Curcumin nanoparticles on the gene expression of the enterococcal surface protein, Esp, involved in biofilm formation of Enterococcus Faecalis. RJMS 2019; 26 (9) :39-46
URL: http://rjms.iums.ac.ir/article-1-5594-en.html
URL: http://rjms.iums.ac.ir/article-1-5594-en.html
Islamic Azad University, Tehran, Iran , dr_kumarss_amini@yahoo.com
Abstract: (3709 Views)
Background: Antibiotic-resistant forms of Enterococcus faecalis, the second leading cause of severe nosocomial infections, have begun to emerge worldwide. Evidences have shown that the Esp expression is related to the primary adherence and biofilm formation of E. faecalis. The present study investigated the effect of curcumin nanoparticles on the Enterococcal surface protein, Esp, involved in biofilm formation of antibiotic-resistant forms of enterococcus faecalis.
Methods: In this study, 60 clinical specimens collected from patients admitted to major hospitals of Tehran, Iran and all specimens were identified by standard bacteriological and biochemical methods. The strains were evaluated for the presence of Esp in E. faecalis by PCR method. After treatment, broth microdilution method and Real-time PCR were used to assess the inhibitory activity of curcumin nanoparticles on biofilm formation and the expression level of Esp gene, respectively.
Results: Twelve E. faecalis harboring Esp gene strains were included. The result of MIC testing and gene expression assay showed that curcumin nanoparticles did not show any inhibitory activity against biofilm formation in clinical isolates of E. faecalis and no significant changes in transcription were observed.
Conclusion: Considering the high prevalence of Esp gene among E. faecalis strains, molecular identification might serve as a potent drug-resistant marker of E. faecalis, as essential elements of E. faecalis for effective infection control program. No significant changes in transcription were detected when the minimal medium was supplemented with curcumin nanoparticles, suggesting that these nanoparticles contribute very little, if at all, to inhibition of the Esp operon.
Methods: In this study, 60 clinical specimens collected from patients admitted to major hospitals of Tehran, Iran and all specimens were identified by standard bacteriological and biochemical methods. The strains were evaluated for the presence of Esp in E. faecalis by PCR method. After treatment, broth microdilution method and Real-time PCR were used to assess the inhibitory activity of curcumin nanoparticles on biofilm formation and the expression level of Esp gene, respectively.
Results: Twelve E. faecalis harboring Esp gene strains were included. The result of MIC testing and gene expression assay showed that curcumin nanoparticles did not show any inhibitory activity against biofilm formation in clinical isolates of E. faecalis and no significant changes in transcription were observed.
Conclusion: Considering the high prevalence of Esp gene among E. faecalis strains, molecular identification might serve as a potent drug-resistant marker of E. faecalis, as essential elements of E. faecalis for effective infection control program. No significant changes in transcription were detected when the minimal medium was supplemented with curcumin nanoparticles, suggesting that these nanoparticles contribute very little, if at all, to inhibition of the Esp operon.
Type of Study: Research |
Subject:
Microbiology
References
1. 1. Toledo-Arana A, Valle J, Solano C, Arrizubieta MaJ, Cucarella C, Lamata M, et al. The enterococcal surface protein, Esp, is involved in Enterococcus faecalis biofilm formation. Appl Environ Microbiol. 2001;67:4538-45.
2. 2. Benes J, Picha D, Kabelkova M, Dzupova O, Horova B,Gabrielova A. Infective endocarditis caused by unusual gram-positive pathogens. Folia Microbiol. 2002;47: 737.
3. 3. Zareba T, Hryniewicz W. Clinical significance of Enterococcus infection. New Med. 1997;4:30-3.
4. 4. Soares RO, Fedi AC, Reiter KC, Caierão J,d'Azevedo PA. Correlation between biofilm formation and gelE, esp, and agg genes in Enterococcus spp. clinical isolates. Virulence. 2014;5: 634-7.
6. 5. Heikens E, Bonten MJ, Willems RJ. Enterococcal surface protein Esp is important for biofilm formation of Enterococcus faecium E1162. J Bacteriol. 2007;189:8233-40.
7. 6. Shepard BD , Gilmore MS. Antibiotic-resistant enterococci: the mechanisms and dynamics of drug introduction and resistance. Microbes Infect. 2002;4: 215-24.
8. 7. Salehi M, Mosavari N, Hosseini F,Mobaraki M. The evaluation of esp and eep genes in Enterococcus strains isolated from clinical urine samples in Tehran. Arak Med Uni J. 2012;15:39-48.
9. 8. Vergis EN, Shankar N, Chow JW, Hayden MK, Snydman DR, Zervos MJ, et al. Association between the presence of enterococcal virulence factors gelatinase, hemolysin, and enterococcal surface protein and mortality among patients with bacteremia due to Enterococcus faecalis. Clin Infect Dis. 2002;35:570-5.
10. 9. Ramadhan A, Hegedus E. Biofilm formation and esp gene carriage in enterococci. J Clin Pathol. 2005;58:685-6.
11. 10. Sava I, Heikens E, Kropec A, Theilacker C, Willems R,Huebner J. Enterococcal surface protein contributes to persistence in the host but is not a target of opsonic and protective antibodies in Enterococcus faecium infection. J Med Microbiol. 2010;59:1001-4.
12. 11. Billström H, Lund B, Sullivan Å, Nord CE. Virulence and antimicrobial resistance in clinical Enterococcus faecium. Int J Antimicrob Agents. 2008;32:374-7.
13. 12. Sharifi Y, Hasani A, Ghotaslou R, Naghili B, Aghazadeh M, Milani M, et al. Virulence and antimicrobial resistance in enterococci isolated from urinary tract infections. Adv Pharm Bull. 2013;3:197.
14. 13. Wilken R, Veena MS, Wang MB,Srivatsan ES. Curcumin: A review of anti-cancer properties and therapeutic activity in head and neck squamous cell carcinoma. Mol Cancer. 2011;10:12.
15. 14. Kocaadam B, Şanlier N. Curcumin, an active component of turmeric (Curcuma longa), and its effects on health. Crit Rev Food Sci Nutr. 2017;57:2889-95.
16. 15. Negi N, Prakash P, Gupta ML,Mohapatra TM. Possible role of curcumin as an efflux pump inhibitor in multi drug resistant clinical isolates of Pseudomonas aeruginosa. J Clin Diagn Res. 2014;8:DC04.
17. 16. Ike Y. Pathogenicity of enterococci. Nihon Saikingaku Zasshi Jap J Bact. 2017;72:189-211.
18. 17. Mohamed JA, Huang DB. Biofilm formation by enterococci. J Med Microbiol. 2007;56:1581-8.
19. 18. Phan TT, See P, Lee ST,Chan SY. Protective effects of curcumin against oxidative damage on skin cells in vitro: its implication for wound healing. J Trauma Acute Care Surg. 2001;51:927-31.
20. 19. Akram M, Shahab-Uddin AA, Usmanghani K, Hannan A, Mohiuddin E,Asif M. Curcuma longa and curcumin: a review article. Rom J Biol Plant Biol. 2010;55:65-70.
21. 20. Zorofchian Moghadamtousi S, Abdul Kadir H, Hassandarvish P, Tajik H, Abubakar S, Zandi K. A review on antibacterial, antiviral, and antifungal activity of curcumin. Biomed Res Int. 2014;2014.
22. 21. Kali A, Devaraj Bhuvaneshwar P, Charles M, Seetha KS. Antibacterial synergy of curcumin with antibiotics against biofilm producing clinical bacterial isolates. J Basic Clin Pharm. 2016;7:93.
23. 22. Jahromi MAM, Rajayi H, Al-Musawi S, Pirestani M, Ramandi MF, Ahmadi K, et al. Evaluation of Antibacterial Effect of Curcumin Loaded Chitosan Nanoparticles. Galen. 2015;5:134-41.
24. 23. Gutierrez JKT, Zanatta GC, Ortega ALM, Balastegui MIC, Sanitá PV, Pavarina AC, et al. Encapsulation of curcumin in polymeric nanoparticles for antimicrobial Photodynamic Therapy. PloS One. 2017;12:e0187418.
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