Volume 25, Issue 7 (10-2018)                   RJMS 2018, 25(7): 70-80 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

zare mongabadi M, farhadi M, torabzadeh khorasani P, hedaiati M. Comparison apoptotic effects of Iranian Artemis (Artemisia scoparia and sieberi) with Taxol on SK _BR3 breast cancer cell line. RJMS 2018; 25 (7) :70-80
URL: http://rjms.iums.ac.ir/article-1-5168-en.html
Karaj Branch, Islamic Azad University, Karaj, Iran. , monafarhadi@yahoo.com
Abstract:   (3976 Views)
Background: Breast cancer is one of the most common cancers among women. Chemotherapy drugs such as taxol often lead to toxicity. Artemisia from Asteraceae family, contain flavonoids such as Artemisin which is one of the most important medicinal plants in the world. Therefore, in the present study the cytotoxic and apoptotic effects of two ethanol extract (Artemisia scoparia, Artemisia siberia) of Artemisia species is studied and compared with Taxol on SK-BR-3 breast cancer cell line.
Methods: In this study, the ethanol extract was prepared. Concentrations of the mentioned ethanol extracts prepared: Artemisia siberia: 60, 30, 15, 7.5 mg/ml and Artemisia scoparia: 1.25, 0.63, 0.31, 0.16 mg/ml. The concentration of Paclitaxel (100 µM) was determined in 24, 48 and 72 hours by MTT assay. The apoptotic effects and induced death were measured using Annexin V and PI.
Results: The MTT results indicated that cytotoxic effects of two Artemisia extracts and Taxol drug are dose-dependent. The obtained IC50 after treatment at 24, 48, 72 hours were (0.55, 0.35, 0.31 mg/ml) and 34.14, 28.02, 18), respectively for Artemisia scoparia and Artemisia siberia. Also, paclitaxel was 56.74, 36.28, and 21.09 (μM). Annexin and PI measurement showed that both extracts, Artemisia Siberia 3.53% and Artemisia scoparia 4.71%, compared to Taxol 5.13% had apoptosis effect and influence on induction of primary and secondary apoptosis, significantly (p<0.05).
Conclusion: Alcoholic extract of Artemisia scoparia with further investigation has the potential for use as a drug for breast cancer treatment.
Full-Text [PDF 1412 kb]   (1526 Downloads)    
Type of Study: Research | Subject: Medical

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Razi Journal of Medical Sciences

Designed & Developed by : Yektaweb