Volume 25, Issue 5 (8-2018)
RJMS 2018, 25(5): 1-8 |
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Seif F, Khoshmirsafa M, Moosavi M, Roudbary M, Khajoei S, Nikfar G, et al . Evaluating the expression of IL-17 and IL-23R genes in Peripheral Blood Mononuclear cells in Rheumatoid Arthritis patients. RJMS 2018; 25 (5) :1-8
URL: http://rjms.iums.ac.ir/article-1-5147-en.html
URL: http://rjms.iums.ac.ir/article-1-5147-en.html
Farhad Seif 
, Majid Khoshmirsafa , Mohammad Moosavi , Maryam Roudbary , Sholeh Khajoei , Golnaz Nikfar , Azam Samei , Mohammadali Bahar , Morteza Hashemzadeh-Chaleshtori , Hedayatollah Shirzad * 
, Majid Khoshmirsafa , Mohammad Moosavi , Maryam Roudbary , Sholeh Khajoei , Golnaz Nikfar , Azam Samei , Mohammadali Bahar , Morteza Hashemzadeh-Chaleshtori , Hedayatollah Shirzad *
1- Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran , shirzad1951@yahoo.com
Abstract: (3733 Views)
Background: Rheumatoid arthritis (RA) is an autoimmune disease caused by accumulation of numerous inflammatory cells in the joints and secretion of various cytokines leading to cartilage and bone damage. IL-17 and IL-23 are inflammatory cytokines that their definite role has not been clearly distinguished in RA pathogenesis. Therefore, this study aimed to investigate the expression and association of IL-17 and IL-23R in Peripheral Blood Mononuclear cells (PBMCs) in RA patients.
Methods: This study was case-control. We gathered peripheral blood from 37 patients with RA and the same number of healthy individuals as a control group. In brief, PBMCs were isolated by Ficoll centrifugation. After RNA extraction and cDNA synthesis, IL-17 and IL-23R expression mRNA levels were determined in PBMCs by real-time PCR technique and Taqman probe method.
Results: The mean±standard deviation of the ages in patient group was 46.86±1.328 yr. and in controls was 44.73±1.392 yr. The expression of IL-17 was increased in RA patients in comparison to healthy controls (P= 0.002). Whereas, after comparison of IL-23R expression in patient and healthy groups, no significant difference was observed (P = 0.22).
Conclusion: In this study, upregulated expression of IL-17 implicated the important role of this cytokine in RA pathogenesis. Therefore, novel therapeutic and more effective strategies can be suggested by further investigations to specifically inhibit IL-17 using monoclonal antibodies (biologic drugs).
Methods: This study was case-control. We gathered peripheral blood from 37 patients with RA and the same number of healthy individuals as a control group. In brief, PBMCs were isolated by Ficoll centrifugation. After RNA extraction and cDNA synthesis, IL-17 and IL-23R expression mRNA levels were determined in PBMCs by real-time PCR technique and Taqman probe method.
Results: The mean±standard deviation of the ages in patient group was 46.86±1.328 yr. and in controls was 44.73±1.392 yr. The expression of IL-17 was increased in RA patients in comparison to healthy controls (P= 0.002). Whereas, after comparison of IL-23R expression in patient and healthy groups, no significant difference was observed (P = 0.22).
Conclusion: In this study, upregulated expression of IL-17 implicated the important role of this cytokine in RA pathogenesis. Therefore, novel therapeutic and more effective strategies can be suggested by further investigations to specifically inhibit IL-17 using monoclonal antibodies (biologic drugs).
Type of Study: Research |
Subject:
Immunology
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