Razi Journal of Medical Sciences

Abstract                                                                                                                                  

Background: Monocytes are a key component of the immune system, which play an important role in inflammatory and innate immune responses. So the isolation of these cells is important in immunology researches and accordingly many methods have been developed for the monocyte isolation. One of the least expensive and simple separation methods is using the ability of these cells to adhere to solid surfaces that were examined in this study.

Methods: In this way, after the isolation of peripheral blood mononuclear cells (PBMCs), monocytes were isolated from cell suspension by using the adhesion ability of these cells to the plastic surface.Isolated monocytes were stained by using specific antibodies labeled with fluorescent dyes (PE, FITC) binding to specific markers on the surface of monocytes CD11b and CD14 and the purity of cells were characterized by flow cytometry.

Results: Flow cytometry results after separation of monocytes shown that 90.2% of cells were expressed two markers CD11b and CD14.

Conclusion: Although there are several monocyte isolation methods, each of them have advantages and disadvantages. One of the least expensive and Simple method of isolation is using adhesion ability of these cells that provides possibility of monocyte isolation with high purity.