Volume 22, Issue 137 (11-2015)                   RJMS 2015, 22(137): 95-101 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ahamdi R, Naghavi E, Molaei S. The effect of waterpipe smoking and chronic immobilization stress on serum creatine kinase and alkaline phosphatase activities in female rats. RJMS 2015; 22 (137) :95-101
URL: http://rjms.iums.ac.ir/article-1-4155-en.html
MSc student, Department of Physiology, Faculty of Science, Azad Isalmic University, Hamedan Branch, Hamedan, Iran , Paradise00751@yahoo.com
Abstract:   (11494 Views)

Background: Various studies indicate that there is association between life style and various enzyme activities. This study was conducted to determine the effects of waterpipe smoking and immobilization on serum creatine kinase and alkaline phosphatase activities in female rats.

Methods: In this experimental study female Wistar rats were divided into control, waterpipe smoking, immobilized, and immobilized waterpipe smoking groups of 7 in each. Exposure to waterpipe smoke was performed 10 times/day for 10min with 5 min resting time interval and animals were chronically immobilized twice a day for 2 hr each time. After 7 weeks, blood samples were collected using cardiac puncture technique and enzyme activity was measured using spectrophotometry. The data were compared statistically between groups using ANOVA.

Results: Serum creatine kinase and alkaline phosphatase activity was significantly higher in immobilized waterpipe smoking than immobilized or waterpipe smoking rats (p=0.009, 0.012 and 0.046, 0.047, respectively). Moreover, the enzyme activity was higher in waterpipe smoking than immobilized rats (p= 0.031 and 0.048, respectively).

Conclusion: Our findings indicate that prevalence of waterpipe smoking and immobilization in our society is a serious threat to health. The necessity to control these factors and publicizing the related information seems inevitable for public health.

Full-Text [PDF 724 kb]   (1464 Downloads)    
Type of Study: Research | Subject: Educational Health

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Razi Journal of Medical Sciences

Designed & Developed by : Yektaweb