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URL: http://rjms.iums.ac.ir/article-1-3927-en.html
Background: Recombinant human Calprotectin is a protein that has inhibitory effect on the growth of cancer. This substance could use as a marker or therapeutic agents for cancer. The goal of this study was to evaluate the anti-tumor property of recombinant human Calprotectin on K562 cell line.
Methods: Recombinant human Calprotectin was cloned and purified in E. coli. Then, different concentrations (1, 5, 10, 20, 40, 80 and 100 µg/ml) were prepared in Roswell Park Memorial Institute (RPMI) and its antitumor property against K562 was assayed by MTT method at the presence of Doxorubicin as positive standard.
Results: Results showed recombinant human Calprotectin has antitumor property against K562 cell line in a concentration and time dependent manner. Statistical analysis revealed recombinant human Calprotectin significantly (P= 0.0231) has inhibitory effect on the growth on K562.
Conclusion: Based on this finding we conclude that recombinant human Calprotectin has anti-cancer properties, but its application needs further studies.
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