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URL: http://rjms.iums.ac.ir/article-1-3901-en.html
Background: Currently, with increasing risk of influenza A virus
epidemics, a lot of studies have been performed. Oseltamivir or Tamiflu (the
neuraminidase (NA) inhibitor) is one of the effective drugs for preventing and
treatment of these viruses. The H274Y mutation is from the most important drug
resistant factors in influenza A viruses. The aim of this study was detection
of Oseltamivir resistant influenza A/H3N2 viruses by 274 position inspection
using Real-time RT-PCR.
Methods: Initially, specific primers and probs for detection of
sensitive and resistant A/H3N2 viruses were designed. The Real-time RT-PCR
assay was performed to detect mutation in 274 position of NA gene.
Results: Of 50 A/H3N2 specimens, all were negative for H274Y
mutation and no resistant viruses were selected.
Conclusion: Quick and accurate recognition of drug resistant mutants
is necessary for effective treatment strategies. Real-time RT-PCR assay is a
rapid operational test which could be performed in the laboratories for
detection of influenza viruses resistant to NA inhibitor.
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