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URL: http://rjms.iums.ac.ir/article-1-3894-en.html
Background: The cancer stem cells (CSCs) are involved in invasion
and metastasis of melanoma, the most lethal and aggressive form of skin cancer.
In the present study, the expression of putative stem cell markers CD133 and
ALDH1 were evaluated in melanoma cell lines and then the cells sorted based on
the expression of these markers.
Methods: In the present study expression of CD133 and ALDH1 was
evaluated in A375 and D10 cell lines using flow cytometry. Then, selected cell
line was sorted up on the expression of selected stem cell marker into positive
and negative populations.
Results: Our results indicated that ALDH1 marker expressed in
55.71 ± 7.88% and 49.88 ± 6.43% in A375 and D10 cell lines, respectively, while
the CD133 only expressed in D10 cells in 26.76 ± 2.83%. Cell sorting was
performed according to ALDH1 expression in both cell lines and CD133 expression
only in D10 cells. The purity of isolated cells was high according to CD133
expression (90% <), whereas the purity of ALDH1+ and ALDH1- populations was
very low.
Conclusion: Findings of this study showed that cell sorting based
on stem cell marker CD133 was suitable in D10 cells, therefore this marker
could be a reliable marker for isolating of CSCs in this cell line.
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