Investigating the effect of ethanolic extract of Ferula assa-foetida's resin on angiogenesis in chick chorioalantoic membrane

Sadoughi, Seyed Damoon; Zafar-Balanezhad, Saide; Baharara, Javad; Nejhad Shahrokhabadi, Khadijeh; Rahbarian, Raheleh; Sepehri-Moghadam, Heshmat

Volume 22, Issue 131 (5-2015) RJMS 2015, 22(131): 80-87 | Back to browse issues page

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Sadoughi S D, Zafar-Balanezhad S, Baharara J, Nejhad Shahrokhabadi K, Rahbarian R, Sepehri-Moghadam H. Investigating the effect of ethanolic extract of Ferula assa-foetida's resin on angiogenesis in chick chorioalantoic membrane. RJMS 2015; 22 (131) :80-87
URL: http://rjms.iums.ac.ir/article-1-3787-en.html

Investigating the effect of ethanolic extract of Ferula assa-foetida's resin on angiogenesis in chick chorioalantoic membrane

Seyed Damoon Sadoughi

, Saide Zafar-Balanezhad

, Javad Baharara

, Khadijeh Nejhad Shahrokhabadi

, Raheleh Rahbarian

, Heshmat Sepehri-Moghadam

Islamic Azad University, Mashhad, Iran , damoon.sadughi@Gmail.com

Abstract: (7495 Views)

Background: Inhibition of angiogenesis is the main goal of cancer treatment and other diseases. Ferula assa-foetida is a medicinal plant with cytotoxic effects. The aim of this study was to investigate the anti-angiogenic effects of Ferula assa-foetida's resin.

Methods: 40 Hy-line fertilized eggs were divided into 4 groups: control, sham, experimental 1 (treated with 100 μg/ml of extract), experimental 2 (treated with 200 μg/ml of extract). On the eighth day of incubation it was created a window in the bottom of the eggs and gelatin sponge was placed on chorioallantoic membrane. 10 microliters of Ferula assa-foetida's resin extract was injected to treated groups. 10 microliters of distilled water was injected to control group and 10 microliters of dimethyl sulfoxide was injected to sham-exposed group. All samples were photographed on the 12th day of incubation and the length and numbers of vessels were analyzed by ANOVA and Tukey’s post hoc (p>0.05)

Results: The mean of number and length for vessels in the control group and mean of number and length in sham-exposed group was not significant. There was a significant decrease in mean number and length of vessels at concentration of 100 and 200 μg/ml of Ferula assa-foetida's resin extract compared with control group.

Conclusion: According to this research, ethanolic extract of Ferula assa-foetida's resin has an inhibitory effect on angiogenesis in chick chorioalantoic membrane. It seems that compounds of Ferula assa-foetida's resin can be used to inhibit angiogenesis in cancer tissues

Keywords: Angiogenesis, Resin of Ferula assa-foetida, Chorioalantoic Membrane, Chick Embryo

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Type of Study: Research | Subject: Biology