ADENYLYL CYCLASE ASSAY TO MEASURE MACROPHAGE CHEMOTACTIC PROTEIN-1 RECEPTOR FUNCTION

Qujeq, D.; Ahmadi, M.

Volume 8, Issue 25 (12-2001) RJMS 2001, 8(25): 283-287 | Back to browse issues page

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Qujeq D, Ahmadi M. ADENYLYL CYCLASE ASSAY TO MEASURE MACROPHAGE CHEMOTACTIC PROTEIN-1 RECEPTOR FUNCTION. RJMS 2001; 8 (25) :283-287
URL: http://rjms.iums.ac.ir/article-1-348-en.html

ADENYLYL CYCLASE ASSAY TO MEASURE MACROPHAGE CHEMOTACTIC PROTEIN-1 RECEPTOR FUNCTION

D. Qujeq

, M. Ahmadi

Abstract: (9566 Views)

Adenylyl cyclase is a membrane-bound enzyme that catalyzes the conversion of ATP to cAMP. The inhibition of adenylyl cyclase was carried out by measuring the ability of the macrophage chemotactic protein-1 to inhibit the forskolin-induced enzyme activity. Measurement of adenylyl cyclase activity was performed according to the procedure described by Wiegn.

Adenylyl cyclase activity in the present macrophage chemotactic protein-1 was decreased compared to that in controls [2.11+/- 0.15(mean +/-SD.)vs. 6.83+/-0.45, activity ( µ mol cAMP/mg protein/min)].

Macrophage chemotactic protein significant reduced adenylyl cyclase activity by 79.9%.

Keywords: 1) Adenylyl cyclase 2) Macrophage chemotactic protein-1

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Type of Study: Research |