Volume 26, Issue 1 (4-2019)                   RJMS 2019, 26(1): 1-9 | Back to browse issues page

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riki M, Tukmechi A, Hajirahimi A, bonyadi F. Evaluation of Inhibitory Effects of heat-killed Lactobacillus casei and Lactobacillus paracasei on human chronic myelocytic leukemia K562 cell line: an in vitro study. RJMS 2019; 26 (1) :1-9
URL: http://rjms.iums.ac.ir/article-1-3299-en.html
Urmia University, Iran , malihe.riki@yahoo.com
Abstract:   (4347 Views)

Background: Probiotics are defined as different microorganisms that may have positive effects on preventing or treatment of special pathologic conditions. Anti-cancer properties of these bacteria have been shown in few studies. The aim of this study was to evaluate anti-tumor effects of heat-killed L. casei and L. paracasei on K562 cell line (chronic myeloid leukemia).
Methods: In this experimental study, bacteria were cultured in special medium at anaerobic condition, then, cells killed at temperature of 100°C. Killed cells were dried by lyophilization, finally were sterilized by autoclaving. Then, different concentrations of heat-killed bacteria (125, 250, 500, 1000 and 2000 μg/ml) were prepared separately. Anti-tumor properties of heat-killed bacteria were determined in vitro at 24, 48 and 72 h with MTT assay.
Results: Our result showed that heat-killed cells of L. casei and L. paracasei statistically (p˂0.05) have Anti-tumor properties in K562. In addition, Anti-tumor properties of heat-killed cells have direct relationship with concentration.
Conclusion: Based on obtained results we conclude that the heat killed Lactobacillus can be used as a potential candidate for further studies on chronic myeloid leukemia treatmentces.
Conflicts of interest: None

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Type of Study: Research | Subject: Biology

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