Volume 26, Issue 1 (4-2019)
RJMS 2019, 26(1): 1-9 |
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riki M, Tukmechi A, Hajirahimi A, bonyadi F. Evaluation of Inhibitory Effects of heat-killed Lactobacillus casei and Lactobacillus paracasei on human chronic myelocytic leukemia K562 cell line: an in vitro study. RJMS 2019; 26 (1) :1-9
URL: http://rjms.iums.ac.ir/article-1-3299-en.html
URL: http://rjms.iums.ac.ir/article-1-3299-en.html
Urmia University, Iran , malihe.riki@yahoo.com
Abstract: (4567 Views)
Background: Probiotics are defined as different microorganisms that may have positive effects on preventing or treatment of special pathologic conditions. Anti-cancer properties of these bacteria have been shown in few studies. The aim of this study was to evaluate anti-tumor effects of heat-killed L. casei and L. paracasei on K562 cell line (chronic myeloid leukemia).
Methods: In this experimental study, bacteria were cultured in special medium at anaerobic condition, then, cells killed at temperature of 100°C. Killed cells were dried by lyophilization, finally were sterilized by autoclaving. Then, different concentrations of heat-killed bacteria (125, 250, 500, 1000 and 2000 μg/ml) were prepared separately. Anti-tumor properties of heat-killed bacteria were determined in vitro at 24, 48 and 72 h with MTT assay.
Results: Our result showed that heat-killed cells of L. casei and L. paracasei statistically (p˂0.05) have Anti-tumor properties in K562. In addition, Anti-tumor properties of heat-killed cells have direct relationship with concentration.
Conclusion: Based on obtained results we conclude that the heat killed Lactobacillus can be used as a potential candidate for further studies on chronic myeloid leukemia treatmentces.
Conflicts of interest: None
References
1. 1. Kwan JM, Fialho AM, Kundu M, Thomas J, Hong CS, Gupta TK, et al. Bacterial proteins as potential drugs in the treatment of leukemia. Leukemia Res; 2009. 33: 1392-99.
2. 2. Mahmoud Abadi A.]Leukemia (Blood cancer)[. Mashhad: Kerdegari Publisher; 2008. P. 11-55. (Persian)
3. 3. Alavi S, Arzanian MT, Moradmand M, Ashraftalesh H. [Prevalence of prognostic factors in children with Acute Lymphoblastic Leukemia admitted to Mofid Childrens]. Iran J Pediatrics; 2005. 15: 237-242. (Persian)
4. 4. Deininger MW, Goldman JM, Melo JV. The molecular biology of chronic myeloid leukemia. Blood; 2000. 96: 3343-56.
5. 5.Ten Bosch GJA, Joosten AM, Kessler JH, Melief CJM, Leeksma OC. Recognition of BCR-ABL positive leukemic blasts by human CD34+ T cells elicited by primary in vitro immunization with a BCR-ABL breakpoint peptide. Blood; 1996. 88: 3522-7.
6. 6. Karpas A, Hayhoe FGJ, Green berger JS, Barker CR, Cawley JC, Lowenthal RM. and Moloney WC. The establishment and cytological, cytochemical and immunological characterization of human haemic cell lines: Evidence for heterogeneity. Leukemia Res; 1977. 1: 35.
7. 7. Kemnitzer W, Drewe J, Jiang S, Zhang H, Zhao J, Crogan-Grundy C. Discovery of 4-aryl-4Hchromenes as a new series of apoptosis inducers using a cell- and caspase-based highthroughput screening assay. 4 structure-activity relationships of fused rings at the 7, 8-positions. J Med Chem; 2007. 50: 2858-64.
8. 8. Wong S, McLaughlin J, Cheng D, Witte ON. Cell context-specific effects of the BCR-ABL oncogene monitored in hematopoietic progenitors. Blood; 2003. 101: 4088-97.
9. 9. Salinas I, Abelli L, Bertoni F, Picchietti S, Roque A, Furones D, et al. Monospecies and multispecies probiotic formulations produce different systemic and local immunostimulatory effects in the gilthead seabream (Sparus aurata L). Fish Shellfish Immunol; 2008. 25(1-2):114-23.
10. 10. Drisko JA, Giles CK, Bischoff BJ. Probiotics in health maintenance and disease prevention. Altern Med Rev; 2003. 8(2):143-55.
11. 11. Tsai YT, Cheng PC, Fun CK, Pan TM. Time-dependent persistence of enhanced immune response by a potential probiotic strain Lactobacillus paracasei subsp Paracasei NTU 101. Inter J Food Microb; 2008. 128: 219-25.
12. 12. Pei-Ren L, Roch-Chui YU, Cheng-Chun C, Echu. Determinations of the antimutagenic activities of several probiotic bifidobacteria under acidic and bile conditions against benzo pyrene by a modified Ames test. Inter J Food Microb; 2004. 93: 249-57.
13. 13. Gackowska L, Michalkiewicz J, Krotkiewski M, Helmin Basa A, Kubiszewska I. Combined effect of different lactic acid bacteria strains on the mode of cytokines pattern expression in human peripheral blood mononuclear cells. J Physiol Pharmac; 2006. 57: 13-21.
14. 14. Lee JW, Shin JG, Kim EH, Kang HE, Yim IB, Kim JY, et al. Immunomodulatory and antitumor effects in vivo by the cytoplasmic fraction of Lactobacillus casei and Bifidobacterium longum. J Vet Sci; 2004. 5(1):41-8.
15. 15. Okawa T, Niibe H, Arai T, Sekiba K, Noda K, Takeuchi S, Hashimoto S, Ogawa N. Effect of LC9018 combined with radiation therapy on carcinoma of the uterine cervix. Cancer; 1993. 72: 1949-54.
16. 16. Chin FL and Tzu MP. In Vitro Effects of Lactic Acid Bacteria on Cancer Cell Viability and Antioxidant Activity. J Food Drug Anal; 2010. 2 (18): 77-86.
17. 17. Bartosch S, Woodmansey EJ, Paterson JC, McMurdo ME, Macfarlane GT. Microbiological effects of consuming a synbiotic containing Bifidobacterium bifidum, Bifidobacterium lactis and oligofructose in elderly persons, determined by real-
18. time polymerase chain reaction and counting of viable bacteria. Clin In Dis; 2005. 40(1): 28-37.
19. 18. Azizpour K. Biochemical characterization of lactic acid bacteria isolated from rainbow trout (Oncorhynchus mykiss) of west Azarbaijan. Res J Biol Sci; 2009. 4(3); 324-6.
20. 19. Kabiri F, Nejati V, Tukmechi A, Delirezh N, Nikbakhsh P. [Inhibitiory properties of cytoplasmic extract of Lactobacilli isolated from common carp intestine on human chronic myelocytic leukemia K562 cell line: an in vitro study]. Tehran Uni Med J; 2011; 68: 691-698. (Persian)
21. 20. Rahmani M, Nguyen TK, Dent P, Grant S. The multikinase inhibitor sorafenib induces apoptosis in highly Imatinib Mesylate-resistant Bcr/Abl+ human leukemia cells in association with signal transducer and activator of transcription 5 inhibition and myeloid cell leukemia-1 down-regulation. Mol Pharmacol; 2007. 72: 788-95.
22. 21. Damia G, Broggini M. Improving the selectivity of cancer treatments by interfering with cell response pathways. Eur J Cancer; 2004. 40(17):2550-9.
23. 22.Zhong N, Chen H, Zhao Q, Wang H, Yu X, Ashley M, et al. Effects of Griseofulvin on Apoptosis Through Caspase-3– and Caspase-9–Dependent Pathways in K562 Leukemia Cells: An In Vitro Study. Curr Thera Res; 2010. 6: 384-97.
24. 23.Xie J, Que W, Chen M, Sun J, Liu T, Liu H, et al. Antitumor effects of cucurmosin in human chronicmyeloid leukemia occur through cell cycle arrest anddecrease the Bcl-2/Bax ratio toinduce apoptosis. Afr J Pharm Pharacol; 2011. 5: 985-92.
25. 24.Chun-Guang W, Jun-Quang Y, Bei.Zhong L, Dan-Ting J, Chong W, Liang Z, et al. Anti-tumor activity of emodin against human chronic myelocytic leukemia K562 cell lines in vitro and in vivo. Eur J Pharmacol; 2010. 627: 33-41
26. 25.Hart AL, Kamm MA. Mechanism of action of probiotics. Recent advances. Inflamm Bowel Dis; 2009. 15: 300-10.
27. 26. Rafter J. The effects of probiotics on colon cancer development. Nut Res Rev; 2004. (17): 277-84.
28. 27. Gorbach SL. Probiotics and gastrointestinal health. A J Gastro; 2000. 95(1): 2-4.
29. 28. Brady LJ, Gallaher DD, Busta FF. The role of probiotic cultures in the prevention of colon cancer. J Nut; 2000. 130: 410-4.
30. 29. Seow SW, Rahmat JNB, Mohamed AAK, Mahendran R, Lee YK, Bay BH. Lactobacillus species is more cytotoxic to human bladder cancer cells than Mycobacterium bovis (Bacillus Calmette-Guerin). J Uro; 2002. 168: 2236-9.
31. 30. Lee JW, Shin JG, Kim EH, Kang HE, Yim IB, Kim JY, et al. Immunomodulatory and antitumor effects in vivo by the cytoplasmic fraction of Lactobacillus casei and Bifidobacterium longum. J Vet Sci; 2004. 5: 41-8.
32. 31. Kim JY, Woo HJ, Kim YS, Lee HJ. Screening for antiproliferative effects of cellular components from lactic acid bacteria against human cancer cell lines. Bio Lett; 2002. 24: 1431-6.
33. 32. Choi SS, Kim Y, Han KS, You S, Oh S and Kim SH. Effects of Lactobacillus strains on cancer cell proliferation and oxidative stress in vitro. Let Ap Microb; 2006. 42: 452-8.
34. 33. Bonyadi F, Nejati V, Tukmechi A, Hasanzadeh SH, Riki M. Evaluation of Apoptotic Effects of Cell Wall and Cytoplasmic Extract from Saccharomyces cerevisiae on K562 Cell Line. Armaghane-Danesh, Yasuj Uni Med Sci J; 2013. 18(9):699-710. (Persian)
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