Razi Journal of Medical Sciences

  Background: TPO gene variations are one of the causes of thyroid autoimmune diseases. The aim of this study was to examine the association between the T1936C, T2229C and A2257C SNPs ) single nucleotide polymorphisms ( of the TPO gene and Anti-TPO level.

  Methods: In this case-control study, 188 individuals (86 males and 102 females), aged 20-80 years, were randomly selected from the Tehran Lipid and Glucose Study population (TLGS). A2257C and T2229C SNPs were detected with RFLP by use of BsrI and Eco57I as restrictive enzymes respectively, while the T1936C SNP was determined with ARMS-PCR.

  Results: In the presence of the C allele of T1936C, Anti-TPO level was significantly increased (CC: 238±43.3, CT: 47.7±15.9, TT: 74.1±11.3 IU/L p 0.002) however, it disappeared after adjustment for sex and age (p 0.059). No significant difference, before and after adjustment, was found in Anti-TPO level in the presence of T2229C SNP (CC: 129.1±24.5, CT: 43.5±12.6, TT: 126.5±13.8 IU/L p 0.196). The association of A2257C with Anti-TPO level was only significant after adjustment for sex and age (p 0.007),and between the ATC ,CTT haplotypes and Anti-TPO level ,the association was significant (p 0.023, 0.021) however, the association it was dominant between CTT and anti-TPO concentration was significant only after adjustment for sex (p 0.014).

  Conclusion: The result of this study, showed age and sex as potential confounders which could modify the association between TPO polymorphisms and Anti-TPO level in a Tehranian population.