Razi Journal of Medical Sciences

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 ABSTRACT

Changes in metabolism of lipids and lipoproteins in diabetes mellitus have been reported. These changes seen to be the cause of cardiovascular diseases. In this study, 143 patients with type two diabetes mellitus were recruited at Yazd diabetic clinic. After 12 to 14 hours fasting, blood was taken from a forearm vein and serum glucose, triglyceride, cholesterol, HDL-c, and VLDL-c were measured. 76 control nondiabetic subjectes were randomly recruited. Serum triglyceride, VLDL-c and HDL-c in patients were significantly higher and serum LDL-c was lower compared with those in normal subjects. Use of glybenclamide tablets and insuline injection reduced serum LDL-c and increased HDL-c significantly in the patients. Duration of diabetes had no effect on serum triglyceride, cholesterol, HDL-c, LDL-c and VLDL-c levels.

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Several polymorphisms within the apo AI-CIII-AIV gene cluster have been detected there is ample evidence about variations at the adjucent apo AI and apo CIII gene loci influence the risk for hypertriglyceridemia and coronary artery disease(CAD). This study was conducted to elucidate the association between two restriction fragment length polymorphisms(RFLPs) of the adjacent apo AI and CIII genes and CAD in Iranian patients. The polymorphisms sites were MspI site in the promoter region of the apo AI gene(G-78A substitution) and SstI site in the apo CIII 3’ untranslated region(G 3175C substitution). Total genomic DNA was prepared from the whole blood of 46 hypertiglyceridemic patients with CAD and 36 normolipidemic Iranian subjects. Amplification of a 355 bp fragment of the promoter region of the apo AI gene and a 280 bp fragment of apo CIII 3’ untranslated region were achieved by polymerase chain reaction(PCR) with specific primers. Genotype distribution and the allele frequencies for the apo AI MspI and apo CIII SstI polymorphi sms were determined and compared in patients and normolipidemic healthy subjects. Dispite the significant differences in plasma lipids concentration between patients and healthy subjects no association between any of two investigated polymorphisms and cornary artery disease was found. This lack of association was held for both allele frequencies and genotype distribution in different sexes.

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    Background & Aim: Propofol is one of the sedative hypnotic drugs used for anesthetizing patients undergoing surgery. This drug is lipid based and therefore long-term infusion of it during prolonged surgeries and in intensive care units has the risk of hyperlipidemia. The aim of the study was to investigate the concentrations of triglyceride, cholesterol, high density lipoprotein, low density lipoprotein, and very low density lipoprotein during administration of propofol and comparing it with that of isoflurane in patients undergoing surgeries in operation rooms of Rasool Akram Hospital. Patients and Methods: The study was an interventional clinical trial all patients undergoing surgeries in Rasool Akram hospital during the years 2004-5 were enrolled into our study. The patients were randomly allocated to two groups first group taking propofol and second taking isoflurane as anesthetic agent. Lipid profile(triglyceride, cholesterol, high density lipoprotein, low density lipoprotein, and very low density lipoprotein) and CRP were checked before, immediately after, and the day after drug administration. For statistical analysis, repeated measurement of ANOVA, Chi square, Paired T-test and Square T-test were used. Results: A total of 97 patients were enrolled into our study, 49 of which took propofol and 48 took isoflurane. The mean operation time was 2.24±0.89 and the mean age of the patients was 38.14±18.99yr. Triglyceride and very low density lipoprotein(VLDL) were the two factors with statistically significant rise after infusion of propofol(P<0.000). Such a change was not detected in isoflurane group this increase was associated with a compensatory decrease the day after operation. Conclusion: Propofol infusion can induce dramatic rises in Triglyceride and VLDL even after short term infusions and therefore special attention must be paid to patients prone to hyper-triglyceridemia and pancreatitis.

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  Background : Brain injury by transient complete global brain ischemia (cardiac arrest) and regional incomplete brain ischemia (ischemic stroke) afflicts a very large number of patients with death or permanent disability. Recent studies suggest that olive extracts suppress inflammation and reduce stress oxidative injury.

  Methods: The aim of the study was to evaluate the effect of dietary Olive Leaf Extract (OLE) on brain cholesterol, cholesterol ester and triglyceride levels as well as brain edema in rat stroke model.

  Five groups, each consisting of 12 male Wistar rats, were studied. First and second groups (control and sham) received distilled water, while three treatment groups received oral olive leaf extract (OLE) for 30 days (50, 75 and 100 mg/kg/day, respectively). Two hours after the last dose, each main group was subdivided to Middle Cerebral Artery Occlusion (MCAO)-operated (n=6) and intact subgroups (n=6) for assessment of neuropathology (brain edema) and brain lipid analysis.

  Results: The brain cholesterol, cholesterol ester and triglyceride levels were greater in experimental groups when compared to controls. Olive leaf extracts reduced brain edema in experimental groups of 75 and 100 mg/kg/day.

  Conclusion: Our data suggest that OLE may be cerebroprotective in a rat model of ischemia-reperfusion. Further work is required to extend these observations.