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Volume 22, Issue 140 (2-2016)
Background: Nowadays one of the most common causes of death is cancer worldwide, which its incidence and mortality rate dramatically increasing in Iran. Saffron herb is locally grown in South Khorasan. The present study reviewed the anticancer properties of saffron stigma on various macromolecule, cell, and animal models. In traditional medicine saffron herb treated many diseases including diabetes, blood pressure and cancer. The modern medical findings indicate that this herb and its active metabolites can be used to produce alternative antitumor drugs. Saffron selectively suppressed growth and proliferation of cancer cells while did not show any inhibitory effect on growth of normal cells. In addition, it reduced the side effects of common therapies. The main components of saffron stigma are monoterpene aldehydes and carotenoides. Its carotenoids, for instance crocin and crocetin, illustrated antioxidant, anticancer and antimutagenic properties more than other metabolites. This review suggested that anti-tumor drugs from saffron stigma can be applied as alternative, safe and promising agents.
Volume 24, Issue 155 (5-2017)
Background: Cold antibody induced platelet clumping is a rare in-vitro phenomenon that causes EDTA independent pseudo thrombocytopenia. This pre-analytical problem leads to a challenge in the clinical laboratory. In such a case platelets tend to easily aggregate in vitro, giving rise to lower platelet counts. The error is detected simply by examining peripheral blood smear. This report describes a 77 year old man with colon cancer who has received 10 units of random donor platelets due to an analytical error.
Case report: Blood sample was collected on EDTA and sodium citrate anticoagulants and counted in different times and various temperatures by cell counter to detecting the cause of thrombocytopenia. The patient had no signs of bleeding. Platelet count was 164000/µl immediately after sampling but declined sharply after 5 minutes. Platelet clumps was seen at the end of peripheral blood smear. We conclude that cold agglutination induced pseudothrombocytopeniais rare phenomenon which resulting in misleading laboratory results.
Conclusion: This condition should be considered in severe thrombocytopenia without abnormal bleeding manifestation. Since the cold coantibodies did not affect platelet function, this patients will not suffer complications from their platelet cold agglutinin, but it could pose a problem under circumstances such as extensive surgery with associated with hypothermia
Volume 30, Issue 7 (10-2023)
Methods: This research is of a fundamental type that was carried out experimentally. First, mouse mammary cancer cells (4T1) purchased from Pasteur Institute of Iran were cultured. Then, the effect of colchicine on apoptosis and related signaling pathways was determined using various tests including cell viability assay, real-time PCR, annexin V and propidium iodide staining, and western blot analysis. In vivo studies were also performed after tumor induction in mice and then their treatment.Effect of colchicineand related signaling pathways were determined using different tests including cell viability assay, real-time PCR, annexin V and propidium iodide staining and western blot analysis. In vivo studies were investigated through tumor induction with 4T1 cells in mice and then their treatment. After tumor formation, the antitumor activity of colchicine was compared in cancer mice. Tumor tissues after isolation, fixation and staining with hematoxylin and eosin were examined with a microscope for the presence of cancer symptoms.
Results: The results showed that colchicine at a concentration of about 300 μg/ml killed 50% of 4T1 cancer cells. Colchicine significantly increased Bax and P53 mRNA expression and decreased Bcl-2 gene expression. In the in vivo study, it was shown that in the tumor tissue of mice treated with colchicine, the evidence of apoptosis and the area of destruction and necrosis was much wider than that of the untreated group. The in vivo study showed that in the group treated with colchicine, the tumor tissue showed more evidence of apoptosis than the control group, and in the treated group, the area of destruction and necrosis was much wider than in the untreated group. In the tumor tissues of mice in the control group (without treatment), the signs of malignancy were more severe than in the group that had been treated, and abnormal mitotic forms were abundantly seen. Also, no metastases to the liver and spleen were observed in the treated group as well as in the control group. Finally, it can be concluded that the oral intake of pure colchicine reduces the size of induced tumors in mice, prevents further proliferation and metastasis, and induces Apoptotic. Studies have shown that tumors that grow faster, such as tumors from 4T1 cancer cells, are more invasive and metastasize to the surrounding tissues, but the cells of these tumors (with a high proportion of dividing cells) are the most sensitive to toxic compounds of the cell cycle, such as colchicine. They have sin. Continuous use of these compounds causes a high percentage of exposed cells to be destroyed. Reduction in the size of tumors and metastasis of cells to other places in mice treated with colchicine was observed in the present study.
Conclusion: Studies have shown that tumors that grow faster, such as tumors from 4T1 cancer cells, are more invasive and metastasize to the surrounding tissues, but the cells of these tumors (with a high proportion of dividing cells) are the most sensitive to They have cell cycle toxic compounds such as colchicine. Continuous use of these compounds causes a high percentage of exposed cells to be destroyed. Reduction in the size of tumors and metastasis of cells to other places in mice treated with colchicine was clearly observed in the present study. Colchicine had an effective role in inhibiting the proliferation of breast cancer cells, and its oral use reduced the size of induced tumors in mice, prevented further proliferation and metastasis, and induced apoptosis. The mechanism of toxicity of colchicine for cancer treatment is still not clear enough. In the present research, the results of all the tests showed that colchicine has an effective role in inhibiting the proliferation of mouse breast cancer cells and apoptosis through increasing the expression of pro-apoptotic genes and proteins Bax and p53, decreasing the expression of anti-apoptotic Bcl2 genes and proteins, and ultimately induces the activation of caspases.
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