Razi Journal of Medical Sciences

Background: Inhibition of angiogenesis is the main goal of cancer treatment and other diseases. Ferula assa-foetida is a medicinal plant with cytotoxic effects. The aim of this study was to investigate the anti-angiogenic effects of Ferula assa-foetida's resin.

Results: The mean of number and length for vessels in the control group and mean of number and length in sham-exposed group was not significant. There was a significant decrease in mean number and length of vessels at concentration of 100 and 200 μg/ml of Ferula assa-foetida's resin extract compared with control group.

Background: Increasing number of patients facing end-organ failure, as well as the therapeutic challenges surrounding allotransplantation, has catalyzed the evolution of tissue engineering and regenerative medicine. The successful recapitulation of development requires choosing an ideal scaffold material as a mediator of biochemical and biophysical signals. The extracellular matrix (ECM) functions as a scaffold for tissue morphogenesis and provides cues for cell proliferation and differentiation. Rabbit decellularized lung tissue was used for fabrication of scaffold.

               Method: In this experimental study, the fabrication of scaffold from rabbit lung tissue was done via snap freezing method, use of sodium dodecyl sulfate (SDS) and cell bleaching. Interactions between scaffold and blastema tissue were evaluated and scaffolds were placed in blastema rings and stored in culture media for 30 days. Sampling of blastema and scaffolding tissues was done for every five days.

            Result: Histological and microscopic analysis confirmed the removal of cells from prepared scaffolds and porosity was done for lung tissue scaffold. The penetration, proliferation and differentiation of cells was also studied when blastema tissues were cultured on scaffold by using different days

         Conclusion: In this study, a three-dimensional natural scaffold was fabricated from lung tissue using SDS treatment. The prepared scaffolds can have an inductive effect on the cell behaviors such as migration, penetration, division, and probable differentiation. However, further studies are necessary to demonstrate the identity and behavior of the cells and scaffold characterization. It seems obtained scaffold after decellularization of lung tissue can be used as suitable model for primary research in lung tissue engineering

Background: Angiogenesis is a complex, active phenomenon, which plays a significant role in physiologic and pathologic processes. Silymarins are combination of flavonoids that have antioxidant and anti-inflammatory effects. In the current research, the effects of silver nanoparticles coated with silymarin on the angiogenesis of the chorioallantoic membrane of chicken fetal and its antioxidant property have been explored.

Methods: Ross Fertilized Chicken eggs were distributed randomly and equally in control group, laboratory control group, and four experimental groups with different densities. In the second day of incubation, some windows were formed on the eggs and, on the eighth day, they were treated. On the twelfth day, some photographs were taken from the subjects’ chorioallantoic membranes, the number and length of the vessels were measured, using the Image J software. Also the Drabkyn test was executed to determine the level of hemoglobin and the obtained results were analyzed as significant (P<0.05), using SPSS software and T-Test. Further, to evaluate the antioxidant activities of silver nanoparticles coated with silymarin, DPPH and ABTS tests were used.

Results: The comparison between the average of the number and length of the vessels in control group and the experimental groups 2, 3, and 4 showed significant reduction. The comparison between the fetal crown-rump length and weight in control group and the experimental groups 3 and 4 showed significant reduction. Also, the average amount of hemoglobin density in the experimental group 4 showed significant reduction, in comparison to the control group. According results from antioxidant assay, the nanoparticles showed dose dependent radical inhibited activity.

Conclusion: In addition, the nanoparticles lead to dose dependent reduction in angiogenesis and increase the removal of free radicals.