Volume 29, Issue 4 (7-2022)
RJMS 2022, 29(4): 214-223 |
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Ethics code: IR.PNU.REC.1398.059
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Shabanloo M, Barzegari A, Naghibi S, Hanani M. Comparison of Three Methods of Aerobic Exercise with Different Intensities on the Expression of Ppary1 Gene in Subcutaneous Adipose Tissue of Vistar Male Rats. RJMS 2022; 29 (4) :214-223
URL: http://rjms.iums.ac.ir/article-1-6339-en.html
URL: http://rjms.iums.ac.ir/article-1-6339-en.html
Department of physical education, Payame Noor University, PO BOX 19395-3697, Tehran, IRAN , ali_barzegari@pnu.ac.ir
Abstract: (266 Views)
Background & Aims: Regular exercise can improve body composition, including reducing fat or increasing muscle mass. Recent findings show that the connection and association of phenotype towards thermogenic brown cells following exercise in rats occurred mainly in subcutaneous stores. One of the sports activity protocols that have recently attracted the attention of sports physiology researchers is periodic exercises with different intensities, which include alternating very intense, intense, and moderate sports activities and active rest periods with very low intensity, which is a very efficient model in terms of It is a time for exercise and it stimulates almost the same metabolic adaptations as regular endurance exercise. Papery protein is one of the key proteins in the conversion of white adipose tissue to brown, but the role of exercises with different intensities on the content of this protein in subcutaneous adipose tissue has not been investigated yet. Therefore, the aim of the present study was the effect of HIIT, HIT, and MIT on the expression of ppary1 and ppary2 genes in the subcutaneous fat tissue of male Wistar rats.
Methods: 32 male Wistar rats were randomly divided into 4 groups of 8: control, medium-intensity continuous training, high-intensity continuous training, and high-intensity intermittent training. 24 hours after the last training session, the rats were anesthetized and the samples of the subcutaneous fat tissue were extracted and analyzed to determine the expression level of pparγ1 genes using RT-PCR laboratory method. One-way analysis of variance and Tukey's post hoc test were used to investigate the intergroup difference of variables with normal distribution. The MIT protocol was implemented in such a way that in the first week, 5 minutes of warming up, 5 minutes of cooling down, and 20 minutes of the main body of the training including running with an intensity of 65% of VO2max at a speed of 20 m/min were performed and the training time was increased weekly, so that in In the sixth week, the training time reached 37 minutes and remained constant until the end of the eighth week. Also, the training speed was unchanged from the first week to the eighth week and was equal to 20 meters per minute. The HIT protocol in the first week included: 5 minutes of warming up, 5 minutes of cooling down, and 20 minutes of running training with 65% VO2max at a speed of 20 m/min and with an increasing incline of the treadmill. The training time was increased weekly so that the training time reached 30 minutes in the sixth week and remained constant until the end of the eighth week. On the other hand, the slope of the treadmill was 2% in the first and second week and 2% was added to the slope every 2 weeks to reach 8% in the seventh and eighth week. Also, the training speed from the first week to the eighth week was also 20 meters per minute and was kept constant.
Results: The results showed that although performing all three training protocols increased the expression of pparγ1 genes compared to the control group, however, no significant difference was observed between the training groups in the changes of pparγ1, while the increase in the expression of this gene in Continuity groups with high intensity were significant. The results of the one-way analysis of variance test showed that there is a statistically significant difference in the expression of the PPARγ1 gene in the subcutaneous fat tissue of the rats of the research groups (P<0.001). The results showed that the expression of the PPARγ1 gene in male Wistar rats of the HIT group has a significant difference (P≥0.001) compared to the control group so that in the HIT group it increased by 0.015442 units compared to the control group. However, no significant difference was observed in the expression of PPARγ1 in the HIT group compared to the MIT and HIIT groups (respectively: p = 0.120, p = 0.948). On the other hand, a significant difference was also observed between the MIT and control groups (p ≥ 0.001), so the MIT group increased by 0.008431 units compared to the control group, while a significant difference between the MIT group was not observed compared to HIT and HIIT groups (respectively: p = 0.120, p = 0.310). Also, the post-test examination in the training groups showed that there is a significant difference in the expression of the PPARγ1 gene between the HIIT and control training groups (p ≥ 0.001) so that in the HIIT group it was 0.013796 units compared to The control group has increased, while no significant difference was observed between the HIIT group compared to the HIT and MIT groups (respectively: p = 0.948, p = 0.310).
Conclusion: PRDM116 protein as a complex protein can act as a two-way switch in the development of brown fat through multiple protein metabolisms. The possible compatibility of this pathway is the very important role of PRDM16 protein and its connection with PPARγ protein for the transformation of immature brown fat cells into mature cells. In this connection, the PPARγ protein, by binding to other proteins called early cell factor-2 (EBF2), leads to the expression and promotion of PRDM16 protein, and then immature brown adipose tissue cells become mature. And then they will be activated by the PGC-1 protein. MIT, HIT, and HIIT exercises increase the expression of the pparγ1 gene, but the increase in the expression of the pparγ1 gene among the HIT training group showed a greater increase than the rest of the training groups, which seems to increase the expression of this gene over time. The duration of the exercise depends.
Type of Study: Research |
Subject:
Sports Physiotherapy
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