Volume 25, Issue 6 (9-2018)
RJMS 2018, 25(6): 90-96 |
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Zare Mehrjerdi A, Zojaji kohan F, Mohammadi N. Comparison of CD34 + fibrocytes in the stroma of CINIII and invasive squamous cell carcinoma of the cervix . RJMS 2018; 25 (6) :90-96
URL: http://rjms.iums.ac.ir/article-1-3674-en.html
URL: http://rjms.iums.ac.ir/article-1-3674-en.html
Iran University of Medical Sciences, Tehran. Iran. , nvmohammadi@tums.ac.ir
Abstract: (6081 Views)
Background: Cervical cancer does not have symptoms in the early stages and almost all invasive carcinomas of the cervix have an earlier stage in which abnormal cells are confined to the epithelium. The intra-epithelial lesions associate with cytological features of invasive cancer. So, it is important to differentiate a cervical intraepithelial neoplasia (CIN) Ш from an invasive cervical squamous cell carcinoma (SCC).
Methods: A list of the patients with a diagnosis of CIN Ш, uterine cervical SCC and normal cervix was extracted from the archives of the department of pathology at two teaching hospitals in Tehran. The blocks were separated and stained slides were prepared. Appropriate blocks for immunohistochemistry (IHC) staining were chosen. The blocks were cut in 4 micrometers thickness and stained for CD34 antigen. The results were interpreted and scored by a pathologist. The data were analyzed through SPSS 17 software using descriptive and chi-square analyses.
Results: The cervical tissue sample of 65 patients were studied; 20 with normal cervix (30.8%), 20 patients with the CIN III (30.8%), and 25 patients with SCC (38.4%) with a mean age of 48.9±3.19. All slides with normal condition (20 cases), 100% of patients with CINIII (20 cases), and 52% of patients with SCC (13 cases) were positive for presence of CD34 + fibrocytes in cervical stroma (p<0.001).
Conclusion: The significant relationship between the loss of CD34 antigen and pathological findings introduces the antigen as a diagnostic test for the detection of changes related to invasive carcinoma in cervix samples.
Methods: A list of the patients with a diagnosis of CIN Ш, uterine cervical SCC and normal cervix was extracted from the archives of the department of pathology at two teaching hospitals in Tehran. The blocks were separated and stained slides were prepared. Appropriate blocks for immunohistochemistry (IHC) staining were chosen. The blocks were cut in 4 micrometers thickness and stained for CD34 antigen. The results were interpreted and scored by a pathologist. The data were analyzed through SPSS 17 software using descriptive and chi-square analyses.
Results: The cervical tissue sample of 65 patients were studied; 20 with normal cervix (30.8%), 20 patients with the CIN III (30.8%), and 25 patients with SCC (38.4%) with a mean age of 48.9±3.19. All slides with normal condition (20 cases), 100% of patients with CINIII (20 cases), and 52% of patients with SCC (13 cases) were positive for presence of CD34 + fibrocytes in cervical stroma (p<0.001).
Conclusion: The significant relationship between the loss of CD34 antigen and pathological findings introduces the antigen as a diagnostic test for the detection of changes related to invasive carcinoma in cervix samples.
Keywords: high grade Intraepithelial neoplasia (CINIII), Invasive squamous cell carcinoma of the uterine cervix(SCC), CD34 antigen, IHC staining
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