Background: We investigated the effects of 12 weeks of voluntary exercise on a running wheel with extraction of flowers Eriobotrya japonica on GDNF in the brain stem induced by 6-hydroxy dopamine.

Methods: In this study, 43 rats were divided into six groups: healthy control, parkinsonian control, training group, parkinsonian training, extract parkinson, training-extract parkinsonian. Training-extract group were housed in individual cages and attached to running wheels during the study period they received 200 mg/kg extract intraperitoneally three times per week. To induce Parkinson, 6-hydroxy dopamine (6-OHDA) (dissolved in saline) was administered intracerebrovenricular (ICV) by a stereotaxic apparatus. GDNF levels in the brain stem were measured by ELISA. Data was analyzed using one-way analysis of variance (ANOVA) and tukey post-hoc test.

Results: There are significant differences in the level of GDNF between training group and parkinsonian control (p=0.015) between parkinsonian training group and parkinsonian control group there was significant difference (p=0.015). GDNF level between training group and parkinsonian training with parkinsonian control was not significant (0. 87, 0.095). There was significant difference between control and parkinsonian control group (p=0.004). Difference between extract-parkinson group and parkinsonian control was not significant (p=0.191). GDNF level difference between extract-training and parkinsonian control was significant (p=0.008). Brainstem GDNF level in extract-parkinson and parkinsonian control (p=0.191) and extract-parkinson and control (P=0.164) was not significant.

Conclusion: Pre-treatment with exercise alone and exercise with extraction of Eriobotrya japonica could prevent the decrease of GDNF level in brainstem against neurotoxic 6-OHDA.