Razi Journal of Medical Sciences

Background: The aim of this research was to determine the Mn superoxide dismutase (Mn SOD) gene expression and the impact of oxidant and inflammatory markers on Mn SOD gene expression in active men and women.

Methods: This research was a semi-experimental with repetitious measurement, and 24 active men (12) and women (12) (21-23 years) voluntarily participated in this research after completing the consent form. Venous blood samples were taken in three stages prior to exercise test, immediately and 3 hr after exercise test (Recovery). SYBER Green PCR Master mix reagent Kit and Real-time PCR method were used for Mn SOD gene expression and Eutoanalyzer for measurement of malondialdehyde (MDA) and lactate dehydrogenase (LDH) levels.

Results: The mRNA Mn SOD did not significantly increase at any time of exercise in men (p≥0.05). But mRNA Mn SOD significant increased in the recovery state in women (= 0.006). In the men group, MDA activity was higher than the basal state in recovery and immediately of exercise (respectively, p= 0.012 and p= 0.014), but significantly increased in recovery state in women (p= 0.002). LDH levels significantly increased immediately and in the recovery state only in women group (p= 0.009 and p= 0.026). Also there was a significant relationship between MDA activity and Mn SOD gene expression in active men (p= 0.001).

Conclusions: From the results of this study it can be found that the oxidants are effective factors on attenuation of Mn SOD gene expression during intensive exercise in active individual.