Razi Journal of Medical Sciences

  Background: The research show that the relationship between antioxidant markers with inflammatory and oxidative stress markers levels changes was not clear , so the purpose of this study is investigation of relationship between total antioxidant status with creatine phosphokinase and hydrogen peroxide in the athlete girls influenced by acute exercise training

  Method: This study was a semi-experimental research with a repeated measures design and 25 athlete girls within the age range of 21-24 years old volunteered to participate in the research after having expressed their consent through a consent form. Blood sample were collected in the three stages pre of GXT ( Graded exercise test) exercise test (grade: 5%, speed: 12 km/h, time: 20 minutes), immediately and 3 h after exercise test ( recovery phase ). Auto analyzer device was used for measurement of total antioxidant status, H₂O₂ and CPK level, also mixed model methods used for statically analysis.

  Result : Total antioxidant status (TAS) concentration was faced with a significance increase after of exercise training (p≤ 0/031) (0.89 ± 0.16 ), but mixed model analysis indicated TAS levels has been reduced in 3h after exercise (p≥ 0/065) ( 0.88 ± 0.15 ). Plasma levels of hydrogen peroxide has not significantly changed after exercise (p≥ 0/255) ( 2.84 ± 1.38 ), and this increase was significant only in the recovery phase (p≤ 0/029) ( 3.04 ± 1.16 ), also CPK levels has been significance increased in both phases after exercise and recovery phase (p₁≤ 0/031, p₂≤0.002) ( 96 ± 18 and 90 ± 19).

  Conclusion: The results of this study show that the immune system in athlete girls by increasing in antioxidants levels, can prevented the tissue injury caused from CPK H₂O₂ in response to acute exercise training . And Contrary to popular belief , CPK enzymes are effective role in stimulate of antioxidant response in athlete girls.