Volume 18, Issue 90 (12-2011)                   RJMS 2011, 18(90): 17-26 | Back to browse issues page

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Abstract:   (7918 Views)


Mellitus (DM). The exact mechanisms of diabetic neurotoxicity are still unknown. Recently oxidative

stress is introduced as one of the factors for diabetics’ neuropathies. Antioxidants also used as

therapeutic agents to reduce the side effects of diabetes. In the present research the antioxidant effects

of total and flavonoid extracts of Cusseta lehmaniana Bunge on high glucose inducted Pc12 were


Central and peripheral neuropathies are the most common side effects of Diabetes


lehmaniana Bungs were prepared

exposed to extracts. Antioxidant activity of extracts, cell viability and apoptosis were studied by

DPPH, MTT, Annexin staining and Western Blotting respectively.

PC12 cell line treated with high glucose was used. Total and flavonoid extract of C.. PC12 cells treated with 6X fold high glucose concentration


Bungs extract increased cell viability after 3 hours pretreatment. DPPH assay demonstrated 50 μg/ml

flavonoid extract can increased radical scavenging inhibits compare with ascorbic acid. Several assays

were used for evaluated of anti apoptotic effect such as: Annexin, SubG1and Western blotting for

expression of Bax protein.

The MTT result showed 50μg/ml of flavonoid extract and 100μg/ml of total C. lehmaniana


lehmaniana Bungs have the potential to protect PC12 cells against glucose neurotoxicity by reducing

apoptosis via increased Bax expression protein.

Based on our findings, it is concluded that both total and flavonoid extract of C.
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Type of Study: Research | Subject: Anatomy

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