Background:
Mellitus (DM). The exact mechanisms of diabetic neurotoxicity are still unknown. Recently oxidative
stress is introduced as one of the factors for diabetics’ neuropathies. Antioxidants also used as
therapeutic agents to reduce the side effects of diabetes. In the present research the antioxidant effects
of total and flavonoid extracts of Cusseta lehmaniana Bunge on high glucose inducted Pc12 were
studied.
Central and peripheral neuropathies are the most common side effects of DiabetesMethods:
lehmaniana Bungs were prepared
exposed to extracts. Antioxidant activity of extracts, cell viability and apoptosis were studied by
DPPH, MTT, Annexin staining and Western Blotting respectively.
PC12 cell line treated with high glucose was used. Total and flavonoid extract of C.. PC12 cells treated with 6X fold high glucose concentrationResults:
Bungs extract increased cell viability after 3 hours pretreatment. DPPH assay demonstrated 50 μg/ml
flavonoid extract can increased radical scavenging inhibits compare with ascorbic acid. Several assays
were used for evaluated of anti apoptotic effect such as: Annexin, SubG1and Western blotting for
expression of Bax protein.
The MTT result showed 50μg/ml of flavonoid extract and 100μg/ml of total C. lehmanianaConclusion:
lehmaniana Bungs have the potential to protect PC12 cells against glucose neurotoxicity by reducing
apoptosis via increased Bax expression protein.
Based on our findings, it is concluded that both total and flavonoid extract of C.| Rights and permissions | |
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