Volume 24, Number 157 (7-2017)                   RJMS 2017, 24(157): 0-0 | Back to browse issues page


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heydarzadeh S, Yaghoubi H. Green Synthesis and Antibacterial Effect of Silver Nanoparticles by Using Extract of Citrus aurantium . RJMS. 2017; 24 (157)
URL: http://rjms.iums.ac.ir/article-1-4565-en.html

PhD Department of Biology, Ardabil Branch, Islamic Azad University, Ardabil, Iran
Abstract:   (607 Views)

Background: There are many different methods for the production of silver nanoparticles (Ag-NPs) because of these nanoparticles can be used for multiple applications. Recently, using of plants in the synthesis of nanoparticles due to a cost effective and eco-friendly approach has been extensively noticed. In this study, the biosynthesis of silver nanoparticles by using extract of Citrus aurantium and its antimicrobial properties has been reported.

Methods: in this study, the extract of Citrus aurantium as the reducing agent is used for the biosynthesis of silver nanoparticles. Reduction of Ag+ ion was obtained after 24 h by using extract of Citrus aurantium in presence of 1 mM silver nitrate (AgNo3) solution. The size, structural, optical and morphological properties of nanoparticles have been analyzed by facility of:  X-ray Diffraction, Field Emission Scanning (Transmission) Electron Microscope.

Result: UV-Vis spectrum exhibit an absorption band at around 400-450 nm suggestion the formation of biological Ag nanoparticles. Size and morphological properties of nanoparticles was performed by TEM which show that particles have spherical shape with diameter of about 5-40 nm. Silver nanoparticles presented the antimicrobial activity against Gram positive and Gram negative bacteria.

Conclusion: The extract of Citrus aurantium has ability of Ag+ ion to reduce to Ag nanoparticles. Also, Ag nanoparticles produced by Green Synthesis had proper antibacterial activity.

     
Type of Study: Research | Subject: Biochemistry

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