Volume 8, Number 25 (12-2001)                   RJMS 2001, 8(25): 300-307 | Back to browse issues page


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Shekarabi M, Oormazdi H, Ghamchili A, Razavi M. A COMPARATIVE STUDY ON THE TWO METHODS OF DIRECT FLUORESCENT ANTIBODY AND KYNION,ACID-FAST STAINING TECHNIQUES ON THE LABORATORY DIAGNOSIS OF CRYPTOSPORLIDIOSIS.. RJMS. 2001; 8 (25) :300-307
URL: http://rjms.iums.ac.ir/article-1-350-en.html

Abstract:   (5586 Views)
Cryptosporidium parvum in humans,as a re-appeared and as one of the four most important ubiquitous enteric pathogen which can cause diarrhare with high level of mortality mainly in the munocompromised patients has stimulated research interested in the world. The quick and accurate laboratory diagnostic report of the pathogen are hlighly important in the prevention of the disease progress. Although kynion acid-fast(AF)staining procedure is accepted in the most clinical laboratories,a comparative study on the preferences  of direct fluorescent (DF) antibody with staining techniques has undertaken in this recent work.During,months,beginning 1/5/1999,the stool samples of 340 apearantly healthy people,185 from patients under chemotherapy and 170 from children under 10 years with chronic diarrhae have been collected for our research studies.The obtained results are as following:1-The sensitivity,specificity and the simplicity of the direct fluorescent (DF) was 100% as compared with kynion acid-fast (AF) technique.2-With regard of minimum detection time unit,each (DF) slide needs one minute, while this time limit for each (AF) slide was 5 minutes.3-By compering the minimum diagnotic level(MDL) by expert technologists in two tecniques, it was 8 for (DF). In other words, at least 60000 oocysts/ml of liquid stools are needed until to be able to detect by (AF) while (MDL) for (DF) was 7500 oocysts/ml.4-By using a single blind test the experience quality of the technologist in two techniques of (DF) and (AF) were evaluated. This test was significantly prefered as compared with (AF) technique.5-In these studies we have found that 0.7% of healthy people ,0.5% of patients under chemotherapy and 4.1% of diarrhaetic children were infected with Cryptosporidium parvum
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