Volume 20, Number 117 (3-2014)                   RJMS 2014, 20(117): 21-29 | Back to browse issues page


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Beikzadeh B, Delirezh N, Habibian R. The comparison of Different Monocytes Isolation Methods with Their Extraction in Magnetic Activated Cell Sorting. RJMS. 2014; 20 (117) :21-29
URL: http://rjms.iums.ac.ir/article-1-2968-en.html

Immunology Urmia University
Abstract:   (2465 Views)

  Background: Peripheral blood is the main source of monocytes to investigate immunology and biology properties. Therefore, isolation methods of these cells are important. In this study, several methods were compared, while the MACS (Magnetic Activated Cell Sorting) technique cannot be used , we offer the most appropriate method with lowest cellular damage and highest purity .

  Methods : methods such as coated flask with Chitosan (7), gelatin (8), plasma (2), gelatin-plasma (2) were compared with MACS (9), From the microscopic views and time of required isolating monocytes (Comparison between the mentioned methods), cell count (9), cell viability (9), the expression of CD14 as a monocyte marker (10) were compared with MACS (9) by using One-way ANOVA and Tukey test.

  Results: The findings indicated that lowest cell count was belonged to controlling group and Chitosan, the highest numbers refer to gelatin-plasma and MACS. Moreover, the highest monocytes CD14 expression was observed in gelatin- plasma and MACS method.

  Conclusions: Hence it can be concluded that each gelatin and plasma methods were better than the control group in the isolation of monocytes. But covering gelatin with plasma has higher purity than other methods to compare with MACS it can be a good method beside that.

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Type of Study: Research | Subject: Immunology

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