Research code: 91486
Ethics code: IR.IAU. TMB.REC. 25.196
Clinical trials code: IR.IAU. TMB.REC. 22.168

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Islamic Azad University , Amir_mirzaie92@yahoo.com
Abstract:   (96 Views)
Background and Aims: Klebsiella pneumoniae is one of the opportunistic pathogens that due to antibiotic resistance and biofilm formation, the use of alternative antimicrobial compounds is recommended against it. The aim of this study was to evaluate the antimicrobial and anti-biofilm activities of aluminum nanoparticles (Al2O3) and analysis of mrkA biofilm gene expression in clinical strains of K. pneumoniae.
Methods: In this experimental study, 100 clinical samples of blood, urine, cerebrospinal fluid, and ulcers were collected and strains of K. pneumoniae were identified using microbiological methods. Subsequently, the antimicrobial and anti-biofilm effects of Al2O3 nanoparticles were investigated using the least inhibitory concentration (MIC) and plate microtiter methods, respectively. Finally, the expression of mrkA biofilm gene in K. pneumoniae strains was studied using Real-Time PCR.
Results: The results of isolation of bacteria showed that out of 100 clinical samples, 20 isolates belonged to K. pneumoniae. Antimicrobial results indicated that K. pneumoniae strains had a MIC between 6.25 and 50 μg/ml. The results also showed that at sub-inhibitory concentrations, Al2O3 nanoparticles had significant anti-biofilm effects (P <0.05). Also, aluminum nanoparticles significantly reduced the expression of mrkA biofilm gene expression in K. pneumoniae strains (P <0.05).
Conclusion: According to the results, Al2O3 nanoparticles had significant antimicrobial and anti-biofilm effects that can be used as an antimicrobial compound candidate with further studies.
     
Type of Study: Research | Subject: Microbiology

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