Volume 28, Issue 5 (8-2021)                   RJMS 2021, 28(5): 0-0 | Back to browse issues page

Research code: 98-3-82-15788
Ethics code: IR.IUMS.REC.1398.1114
Clinical trials code: 0

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minaeian S, Bahadorizadeh L, Taheri Tinjani R, Shamlou Mahmoudi F, Khazaie F, Alidoust L, et al . Evaluation of phenotypic resistance pattern of gram-negative bacteria isolated from sputum of patients admitted to intensive care units of Firoozabadi hospital. RJMS. 2021; 28 (5)
URL: http://rjms.iums.ac.ir/article-1-6586-en.html
Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran. , sara.minaeian@gmail.com
Abstract:   (428 Views)
Background and Objectives: Nosocomial infection is the most common phenomena in hospital and is one of the important factors influencing the increase in mortality, hospital duration and costs. The use of long term antibiotic therapy leads to emerge multi drug resistance in patients who admitted to intensive care unit (ICU). Transmission of MDR bacteria from one patient to the other patient by treatment staff is another challenge in nosocomial infections especially in intensive care units. Currently one of the most important concerns of World Health Organization (WHO) is the ineffectiveness of most antibiotics against resistant bacteria. Therefore the prevalence of MDR pathogens around the world is gradually increasing. If this trend continues, it can be very difficult to control these bacteria in the future. Therefore, periodic monitoring of the type of bacteria causing nosocomial infections and their resistance pattern is special importance for any medical center. The aim of this study is to investigate the phenotypic resistance pattern of gram-negative isolates, isolated from sputum of patients admitted to the intensive care units of Firoozabadi Hospital.
Methods: In this study, 147 gram-negative isolates were isolated from sputum of patients admitted to the intensive care units of Firoozabadi Hospital and were identified by microscopic and biochemical methods. Then, for all isolates, antibiogram test was performed by Kirby-Bier method (Disk Diffusion) according to CLSI 2019 standard. 0.5 McFarland from fresh bacteria was prepared. Autoclaved Mueller Hinton agar plate was poured into the sterile Petri dishes. Each bacterium was inoculated on the surface of plates. Each antibiotic disc was placed on an agar plate and they were incubated at a temperature of 37 °C for 18–24 h. Inhibition zones were repeatedly measured three times and mean values of zone diameter are reported.
Results: The results of this study showed that the highest rate of infection related to Acinetobacter baumannii isolates 68(46.3%), (Klebsiella pneumonia isolates 52 (35.4%), Pseudomonas aeruginosa isolates 20 (13.6%), E.coli isolates 4 (2.7 %), Citrobacter divercus isolates 2 (1.4%) and Citrobacter freundii isolate 1 ( 0.7% ) respectively.  The highest percentage of resistance among all isolates were found in piperacillin (92.5%) and cefepime (91.8%), respectively and the lowest percentage of resistance in all isolates was seen in ciprofloxacin (74.8%). In addition to the highest percentage of resistance in Acinetobacter baumannii isolates was related to piperacillin (96.96%) and ciprofloxacin (96.55%), in Klebsiella pneumoniae isolates was seen in ciprofloxacin (94.73%) and piperacillin (94%), in Pseudomonas aeruginosa isolates were found in ciprofloxacin (100%), imipenem (95%) and cefepime (95%) and in E.coli isolates was seen 100% resistance to ceftriaxone, ciprofloxacin, ceftazidime, piperacillin and cefepime. Among 147 isolates, 44.21% of Acinetobacter baumannii isolates, 32.65% of Klebsiella pneumonia isolates, 12.92% of Pseudomonas aeruginosa isolates and 2.72% of E.coli isolates were multi drug resistance.
Conclusion: Numerous research and reports suggest that the staff of intensive care units (ICUs) have a lot of challenges to control hospital infection. Bacteria rapidly become resistant if the conditions are right for them. Due to the multi drug resistance bacterial infection in the wards of hospital especially in intensive care units, the periodic monitoring of the microorganism type and their pattern of antibiotic resistance is very necessary and essential for mange the nosocomial infection. The reason for the difference in the statistics data obtained in this studies compared to other similar studies can be due to differences in the geographical and temporal location of studies.
Type of Study: Research | Subject: Microbiology

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