Volume 27, Issue 12 (2-2021)                   RJMS 2021, 27(12): 0-0 | Back to browse issues page

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ijadi M, omidifar A, ghaedi H, kazerouni F. Comparison of ANGPTL4 gene expression in PBMC cells of type 2 diabetic patients and healthy subjects. RJMS. 2021; 27 (12)
URL: http://rjms.iums.ac.ir/article-1-5773-en.html
Associate professor of clinical biochemistry, Department of Medical Laboratory Sciences, school of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran , abol711371@gmail.com
Abstract:   (95 Views)
Introduction: Type two diabetes mellitus (T2DM) is recognized as a metabolic disorder related to various complications such as heart disease, renal disease, and ischemic stroke. The ANGPTL4 is recognized as crucial protein that modulates glucose homeostasis and recent data suggest that ANGPTL4 may be correlated with the risk of type two diabetes. We sought to investigate ANGPTL4 expression in type 2 diabetic patients compared to healthy participants. Materials and Methods: We recruited 50 patients with type 2 diabetes mellitus and 50 healthy individuals that referred to Shohadayeh Tajrish Hospital. FBS and insulin circulating levels were measured using Hitachi autoanalyzer and ELISA method respectively. We separated mononuclear leukocytes (PBMC) using a density gradient process following ficoll solution. Upon extraction of PBMC, we extracted RNA using Gene All kit. Then Real-time PCR method performed to assess the transcript levels of ANGPTL4. Results: The ANGPTL4 gene expression in type 2 diabetic group was decreased compared with the healthy participants, but it was not statistically significant. (P <0.065). Overall, our results demonstrate that there is a positive correlation between FBS and ANGPTL4 expression (r= 0.214, p<0.0067). Conclusion: The level of expression of ANGPTL4 in subjects with type 2 diabetes has diminished. It seems that despite studies on the relationship between ANGPTL4 and type 2 diabetes, we unable to describe the possible relationship and mechanism of ANGPTL4 in metabolic pathways. Further studies are needed to investigate this subject.

     
Type of Study: Research | Subject: Clinical Biochemistry

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