Volume 26, Issue 6 (9-2019)                   RJMS 2019, 26(6): 84-94 | Back to browse issues page

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Ghadimi F, Mirzaie A, Arasteh J. Antibacterial and cytotoxicity of synthesized silver nanoparticles using Erica carnea extract on breast cancer cell line (MCF-7) and analysis of its apoptotic effects. RJMS 2019; 26 (6) :84-94
URL: http://rjms.iums.ac.ir/article-1-5643-en.html
Islamic Azad University , a.mirzaie@riau.ac.ir
Abstract:   (3774 Views)
Background: Recently, metal nanoparticles, especially silver nanoparticles (AgNPs), have attracted the attention of researchers due to their biological properties. The aim of this study was to investigate the biological synthesis of AgNPs using Erica carnea extract and its antibacterial, and cytotoxic effects on breast cancer cell line (MCF-7).
Methods: In this experimental study, the AgNPs were synthesized by adding E. carnea extract to silver nitrate. Subsequently, its physical and chemical properties were determined by ultraviolet spectrophotometry (UV/Vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Subsequently, its antimicrobial, cytotoxic and apoptotic effects were investigate using minimum inhibitory concentration (MIC), MTT and flow cytometry (Annexin-V/PI kit) methods, respectively.
Results: The UV/Vis adsorption results showed that the synthesized AgNPs had a maximum absorbance at 420 nm. In addition, SEM and TEM results indicated that the AgNPs was spherical and had an average size of 10.67 nm. The results of MIC show that the AgNPs had significant effect on Gram negative bacteria. The results of cell cytotoxicity at 125.3 to 100 μg/ml concentrations showed that the IC50 value was 7.14 microgram/mL. Apoptotic results showed that AgNPs induced 60% apoptosis in the MCF-7 cell line.
Conclusion: Based on results of this study, it can be concluded that the AgNPs synthesized by the E. carnea extract had significant antibacterial and anticancer effects and can be used as a drug candidate for the future.
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Type of Study: Research | Subject: Biology

References
1. 1. Salata O. Applications of nanoparticles in biology and medicine. J Nanobiotechnol; 2004. 2(1):3-10.
2. 2. Sánchez-Moreno P, de Vicente J, Nardecchia S, Marchal JA, Boulaiz H. Thermo-Sensitive Nanomaterials: recent advance in synthesis and
3. biomedical applications. Nanomaterials (Basel); 2018. 8(11):10-16.
4. 3. Ge L, Li Q, Wang M, Ouyang J, Li X, Xing MM. Nanosilver particles in medical applications: synthesis, performance, and toxicity. Int J Nanomed; 2014. 9(1):2399-407.
5. 4. Barkat MA, Harshita, Beg S, Naim MJ, Pottoo FH, Singh SP, Ahmad FJ. Current progress in synthesis, characterization and applications of silver nanoparticles: precepts and prospects. Recent Pat Antiinfect Drug Discov; 2018.13(1):53-69.
6. 5. Mohammed AE, Al-Qahtani A, Al-Mutairi A, Al-Shamri B, Aabed KF. Antibacterial and cytotoxic potential of biosynthesized silver nanoparticles by some plant extracts. Nanomaterials (Basel); 2018. 8(6):36-42.
7. 6. Rojas K, Stuckey A. Breast cancer epidemiology and risk factors. Clin Obstet Gynecol; 2016. 59(4):651-672.
8. 7. Tao Z, Shi A, Lu C, Song T, Zhang Z, Zhao J. Breast cancer: epidemiology and etiology. Cell Biochem Biophys; 2015. 72(2):333-8.
9. 8. Ghoncheh M, Pournamdar Z, Salehiniya H. Incidence and mortality and epidemiology of Breast cancer in the world. Asian Pac J Cancer Prev; 2016. 17(S3):43-6.
10. 9. Ramaswami R, Harding V, Newsom-Davis T. Novel cancer therapies: treatments driven by tumour biology. Postgrad Med J; 2013. (1057):652-8.
11. 10. Spicer J1, Chowdhury S, Harper P. Targeting novel and established therapies for non-small cell lung cancer. Cancer Lett; 2007. 250(1):9-16.
12. 11. Ullah Khan S, Saleh TA, Wahab A, Khan MHU, Khan D, Ullah Khan W, et al. Nanosilver: new ageless and versatile biomedical therapeutic scaffold. Int J Nanomedicine; 2018. 13:733-762.
13. 12. Buttacavoli M, Albanese NN, Di Cara G, Alduina R, Faleri C, Gallo M. Anticancer activity of biogenerated silver nanoparticles: an integrated proteomic investigation. Oncotarget; 2017. 9(11):9685-9705.
14. 13. Zhang XF, Liu ZG, Shen W, Gurunathan S. Silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches. Int J Mol Sci; 2016. 17(9).
15. 14. Aboelfetoh EF, El-Shenody RA, Ghobara MM. Eco-friendly synthesis of silver nanoparticles using green algae (Caulerpa serrulata): reaction optimization, catalytic and antibacterial activities. Environ Monit Assess; 2017.189(7):349.
16. 15. Ajitha B, Ashok KRY, Sreedhara RP. Green synthesis and characterization of silver nanoparticles using Lantana camara leaf extract. Mater Sci Eng C; 2015. 49(1):373–381.
17. 16. RHS A-Z encyclopedia of garden plants. United Kingdom: Dorling Kindersley. 2008. p. 1136.
18. 17. Behboodi S, Baghbani-Arani F, Abdalan S, Sadat Shandiz SA. Green engineered biomolecule-capped silver nanoparticles fabricated from Cichorium intybus extract: in vitro assessment on apoptosis properties toward human breast cancer (MCF-7) cells. Biol Trace Elem Res. 2019.187(2):392-402.
19. 18. Mohammad Yasir, Jaspreet Singh, Manish Kumar Tripathi, Pushpendra Singh, Rahul Shrivastava.Green synthesis of silver nanoparticles using leaf extract of common Arrowhead Houseplant and its anticandidal activity. Pharmacogn Mag; 2017. (Suppl 4): S840–S844.
20. 19. Escárcega-González CE, Garza-Cervantes JA, Vázquez-Rodríguez A, Montelongo-Peralta LZ, Treviño-González MT, Díaz Barriga Castro E. In vivo antimicrobial activity of silver nanoparticles produced via a green chemistry synthesis using Acacia rigidula as a reducing and capping agent. Int J Nanomedicine; 2018. 13:2349-2363.
21. 20. Khorrami S, Zarrabi A, Khaleghi M, Danaei M, Mozafari MR. Selective cytotoxicity of green synthesized silver nanoparticles against the MCF-7 tumor cell line and their enhanced antioxidant and antimicrobial properties. Int J Nanomed; 2018. 27;13:8013-8024.
22. 21. Baharara J, Ramezani T, Divsalar A, Mousavi M, Seyedarabi A. Induction of Apoptosis by Green Synthesized Gold Nanoparticles Through Activation of Caspase-3 and 9 in Human Cervical Cancer Cells. Avicenna J Med Biotechnol; 2016. 8(2):75-83.
23. 22. Patil MP1, Kim GD. Eco-friendly approach for nanoparticles synthesis and mechanism behind antibacterial activity of silver and anticancer activity of gold nanoparticles. Appl Microbiol Biotechnol; 2017. 101(1):79-92.
24. 23. Mashwani ZU, Khan MA, Khan T, Nadhman A. Applications of plant terpenoids in the synthesis of colloidal silver nanoparticles. Adv Colloid Interface Sci; 2016. 234:132-41.
25. 24. Moshfegh A, Jalali A, Salehzadeh A, Sadeghi A. Biological synthesis of silver nanoparticles by cell-free extract of Polysiphonia algae and their anticancer activity against breast cancer MCF-7 cell lines. Micro & Nano Letters; 2019. 14: 581-584.
26. 25. Salehi S, Sadat Shandiz SA, Ghanbar F, Darvish MR, Shafiee Ardestani M, Mirzaie A, JafariM.Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial partextract and assessment oftheir antioxidant, anticancerand antibacterial properties. Int J Nanomed; 2016. 11:1835–1846.
27. 26. Khalil M, Gurbuz M, Simone TM, Mousa SA. Nanoparticles and cancer therapy: a concise review with emphasis on dendrimers. Int J Nanomedicine; 2009.4:1-7.
28. 27. Vijayakumar M. Biosynthesis characterisation and anti-bacterial effect of plant mediated
29. 28. Silver nanoparticles using Artemisia nilagirica. Ind Crop Prod; 2013. 41: 235-42.
30. 29. Patra JK, Baek KH. Biosynthesis of silver nanoparticles using aqueous extract of silky hairs of corn and investigation of its antibacterial and anticandidal synergistic activity and antioxidant potential. IET Nanobiotechnol; 2016.10(5):326-333.
31. 30. Foldbjerg R, Dang DA, Autrup H. Cytotoxicity and genotoxicity of silver nanoparticles in the human lung cancer cell line, A549. Arch Toxicol; 2011. 85(7):743-50.
32. 31. Mousavi B, Tafvizi F, Zaker Bostanabad S. Green synthesis of silver nanoparticles using Artemisia turcomanica leaf extract and the study of anti-cancer effect and apoptosis induction on gastric cancer cell line (AGS). Artif Cells Nanomed Biotechnol; 2018. 23:1-12.
33. 32. Salehzadeh A, Sadat Shandiz A, Naeemi AS. Cytotoxicity effectiveness of biosynthesized silver nanoparticles on breast cancer T47D cell line, using macro algae Laurencia caspica extract. Sci J Ilam Uni Med Sci. 2018. 26(1):52-61.
34. 33. Zhu B, Li Y, Lin Zh, Zhao M, Xu T, Wang Ch, et al. Silver nanoparticles induce HePG- 2 cells apoptosis through ROS-mediated signaling pathways. Nanoscale Res Lett; 2016. 11:198-206.

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