Volume 26, Issue 2 (5-2019)
RJMS 2019, 26(2): 74-84 |
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Jozaie A, Movahedi M, Khosravi M, Golab F. The effects of adenosine injection after of brain ischemia reperfusion injury on gene expression of NF-kB/p65 and activity level of ROS in male Wistar rats. RJMS 2019; 26 (2) :74-84
URL: http://rjms.iums.ac.ir/article-1-5495-en.html
URL: http://rjms.iums.ac.ir/article-1-5495-en.html
Islamic Azad University, North Tehran, Tehran, Iran , m.movahhedi@iau-tnb.ac.ir
Abstract: (3579 Views)
Background: Unit of p65 is one of the subunits of NF-κB and its phosphorylation by stress oxidative causes activation of NF-κB. The aim of present study was to investigate the effects of adenosine injection after brain ischemia reperfusion injury on gene expression of NF-κB /p65 and Reactive Oxygen Species (ROS) in hippocampus tissue of male wistar rats.
Methods: 40 male wistar rats were randomly divided into four groups, healthy control (n=10), ischemia reperfusion (n=10), ischemia reperfusion + adenosine dose of 0.1 mg/kg (n=10) and ischemia reperfusion + adenosine dose of 0.4 mg/kg (n=10). The animals were subjected to ischemia by the ligation of common carotid arteries for 45 min. Intraperitoneal injection of adenosine was done after 24 h ischemia in rats. The gene expression of NF-κB /P65 and ROS activity were examined by Real time-PCR and Spectroflourimeter, respectively. One-way ANOVA and Bonferroni post hoc test were used to analysis the data. The significant level was set at p<0.05.
Results: The results showed that ischemia induced significant increase in gene expression of NF-κB /p65 and ROS activity compared to control group (p<0.05). Also, both dose of adenosine resulted in significant decrease in gene expression of NF-κB /p65 and ROS activity compared to ischemia group (p<0.05).
Conclusion: Brain ischemia reperfusion causes the stress oxidative. It appears that injection of adenosine is effective in reduction of stress oxidative and inflammation due to brain ischemia reperfusion.
Methods: 40 male wistar rats were randomly divided into four groups, healthy control (n=10), ischemia reperfusion (n=10), ischemia reperfusion + adenosine dose of 0.1 mg/kg (n=10) and ischemia reperfusion + adenosine dose of 0.4 mg/kg (n=10). The animals were subjected to ischemia by the ligation of common carotid arteries for 45 min. Intraperitoneal injection of adenosine was done after 24 h ischemia in rats. The gene expression of NF-κB /P65 and ROS activity were examined by Real time-PCR and Spectroflourimeter, respectively. One-way ANOVA and Bonferroni post hoc test were used to analysis the data. The significant level was set at p<0.05.
Results: The results showed that ischemia induced significant increase in gene expression of NF-κB /p65 and ROS activity compared to control group (p<0.05). Also, both dose of adenosine resulted in significant decrease in gene expression of NF-κB /p65 and ROS activity compared to ischemia group (p<0.05).
Conclusion: Brain ischemia reperfusion causes the stress oxidative. It appears that injection of adenosine is effective in reduction of stress oxidative and inflammation due to brain ischemia reperfusion.
Type of Study: Research |
Subject:
Biochemistry
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