Volume 25, Issue 168 (6-2018)                   RJMS 2018, 25(168): 0-0 | Back to browse issues page

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Ghaffari R, Nasiri Khalili M A, Khodadadi S, Riazi G. PEGylation of Human growth hormone with Methoxy PEG Succinimidyl Carbonate and study of its half-life and stability. RJMS. 2018; 25 (168)
URL: http://rjms.iums.ac.ir/article-1-5170-en.html
Assistant Professor
Abstract:   (193 Views)
 
Abstract. Human growth hormone (hGH), a single polypeptide consisting of 191 amino acid residues, is a 22 kDa protein that secreted from the pituitary gland.Due to important biological functions and therapeutic applications of hGH, the use of growth hormone for the treatment of obesity, short stature, burns and the importance of this hormone for athletes, use of this drug is increasing. Nowadays, low half-life of hGH is a major challenge associated with the development of this drug in the world. Chemical modification is one of the best strategies for improving half-life and stability. Several studies have shown that enzymatic or nonenzymatic chemical modifications of specific residues of a protein would result in significant changes in its physicochemical properties. PEGylation is an effective approach to increase half-life and stability of biopharmaceutical proteins. Polyethylene glycol (PEG) with the general formula HO-(CH2CH2O)n-CH2CH2OH is a family of long chain polymers made up of ethylene glycol subunits. Due to high aqueous solubility, PEG polymer is considered as a versatile candidate for pharmaceutical protein conjugation. Accordingly,in this study the PEGylation of human growth hormone and its plasma half-life and stability were carrid out.
Methods: hGH was PEGylated by using Methoxy PEG Succinimidyl Carbonate (mPEG-SC) at 8 °C for overnight.  In this study, SDS-PAGE , Dynamic  light scattering (DLS), and ELISA techniques were used to prove PEGylation reaction.
Results: The SDS-PAGE results showed high yield PEGylation of hGH, under aqueous conditions. The zeta-potential, as an indicator of the Surface charge of PEGylated hGH, was analyzed by Dynamic light scattering (DLS) technique. The ELISA results shown the increase half-life of pegylated hGH to 12 times.
Discussion:  In the present study, we demonstrated that the PEGylation of lysine and/or histidine residues significantly increased the half-life of the human growth hormone.
     
Type of Study: Research | Subject: Biochemistry

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