Razi Journal of Medical Sciences

fa اثر هشت هفته تمرین منتخب بر سطوح سرمی فاکتورهای التهابی بازیکنان نخبه واترپلو The Effect of Physical Activity on Serum Levels of Anti-Inflammatory Factors in Athletes فیزیولوژی انسانی Human Physiology پژوهشي Research <div style="text-align: justify;"><a name="_Hlk184301929">زمینه و هدف:</a> بهترین تمرین برای ثبت بهترین رکورد، تمرینی است که با توجه به نیازهای فیزیولوژیکی همان رشته ورزشی طراحی شده و بیشترین شباهت را به شرایط رقابتی آن داشته باشد. هدف از انجام تحقیق حاضر تاثیر هشت هفته تمرین منتخب بر سطوح سرمی فاکتورهای التهابی بازیکنان نخبه واترپلو بود. روش کار: برای انجام تحقیق نیمه آزمایشی حاضر 30 واترپلو کار مرد (با میانگین سنی 3±23 سال) به صورت تصادفی به دو گروه 15 نفری تجربی (تمرین منتخب واترپلو) و کنترل (تمرین سنتی واترپلو)  تقسیم شدند. تمرین منتخب واترپلو به مدت هشت هفته و هر هفته 6 جلسه با شدت 80 درصد حداکثر ضربان قلب انجام شد. جهت بررسی سطوح سرمی  IL-10، TNF-α، 5  میلی لیتر خون وریدی در مرحله پیش و پس آزمون گرفته شد و با روش الایزای ساندویچی سنجش گردید. جهت مقایسه میانگینهای اندازهگیری شده از آزمون تی (t-test) در سطح معنی داری 05/0 P< استفاده شد. یافته‌ها: نتایج نشان داد که گروه تجربی  نسبت به گروه کنترل افزایش معناداری در میزان IL-10 داشته‌اند، اما در میزان TNF- α تغییر معناداری مشاهده نشد. نتیجه گیری: نتایج تایید کننده تاثیر تمرینات واترپلو بر فاکتورهای التهابی ورزشکاران واترپلو می‌باشد؛ با این حال جهت روشن شدن تاثیر لازم است تحقیقات بیشتری در این زمینه انجام شود.</div> <div style="text-align: justify;">Background & Aim: New progress has been made in more than one field of sports science. One of the most important and current topics in the development of this field is the science of practice. In recent years, coping with a sedentary and car-free life is of particular importance to societies today, as doing exercise programs has become an undeniable necessity for disease prevention and quality of life. Noses. According to the design of exercise science, the best type of exercise to record the best record is the exercise that becomes the same sport according to the physiological needs and had the most similarity with its competitive conditions .Therefore, better selection and design in special training for swimming and water polo training needs to be found due to the competitive competition during the working group, which needs to maintain higher physical and motor fitness. In this regard, exercise immunology studies have focused on key components of immune functions such as immune cells, immunoglobulins, glutamines and messenger molecules (cytokines) and the effects of environmental, nutritional and exercise factors. Meanwhile, interleukin10 attracts a lot of attention because on the one hand it is in the post-exercise period that increases insulin release and on the other hand it is associated with obesity and decreased insulin function. There are conflicting findings about the appropriate intensity to reduce inflammatory factors and observe the anti-inflammatory effects of physical activity. Exercise with moderate and high intensity and duration in the training period has a greater effect on inflammatory variables and reduces inflammatory factors, and lower intensities have less effects. Although little research has been done on the relationship between intermittent exercise and changes in interleukin and interleukin10; But mainly in them, only nutritional and medical interventions are emphasized and more on patients. In the present study, the changes of these factors in relation to the adaptations of intense periodic training on water polo athletes without nutritional and pharmacological considerations are investigated. Finally, it seeks to answer the question, what effect does eight weeks of selected training have on the serum levels of Interleukin-10 and TNF-α elite water polo players? Methods: The aim of this study was to evaluate the effect of eight weeks of selected training on serum levels of IL-10,TNF-α of elite water polo players. For this purpose, 20 male water polo players (with a mean age of 23 years) were randomly divided into two groups of 10 experimental (selected water polo training) and control (traditional water polo training). Selected water polo training was performed for eight weeks and 6 sessions per week. To control the intensity of training, used percentage of maximum heart rate (80-100% MHR); Also, the intensity of training was increased by 5% every week. In this study, To evaluate the serum levels of IL-10,TNF-α 5 cc of venous blood was taken in the pre-and post-test stages and measured by sandwich ELISA method. To compare the measured means,t-test was used at a significant level of P <0.05. Results: The results showed that eight weeks of selected water polo training caused significant changes in IL-10 levels of elite water polo players (P <0.05), however, there were no significant changes in TNF-α levels. Conclusion: The results of the present study showed that eight weeks of selected water polo exercises had a significant increase in serum IL-10 level in the experimental group but these results did not show a significant increase in TNF-α serum level in the experimental group. Consistent with our research results, many studies have reported an increase in anti-inflammatory cytokines after a period of exercise (29,30,31). Among the studies that examined the effect of exercise on serum interleukin-10 levels; According to the results of our study, Baldusi et al. (2010) in a study measured the effect of one year of aerobic and resistance training on diabetic patients and observed a significant increase in interleukin 10 in one of their research groups compared to the control group. The results of some studies have also shown that obesity causes the production of TNF-α, leptin and proinflammatory cytokines such as 4-IL and 5-IL in patients with asthma (8). Regarding studies close to the present study, we can refer to Nikzad et al. (2017), who showed that twelve weeks of swimming training (on mice). Has decreased the amount of proinflammatory cytokine 6-IL. Ebrahimpour et al. (2017,) also showed that 8 weeks of endurance swimming training increased the inflammatory effects of breast cancer by increasing interleukin-10 and decreasing interleukin-6 in heart tissue(3). Also, the research of Kapomakio et al. (2011) on the effect of physical exercise on swimmers, an increase in interleukin-6 expression levels and no change in interleukin-6 receptor in the training group compared to the control group was observed. Rihaneh et al. In 2013 achieved almost the same findings, so that in the studies of these researchers, 6-IL was significantly increased as a result of exercise(25). our results showed that eight weeks of selected water polo training caused significant changes in plasma levels of interleukin-10 elite water polo players, however, it could not cause significant changes in plasma levels of TNF-α. </div> واترپلو, IL-10, TNF- α Water Polo, Interleukin 10, TNF-α, 1 9 http://rjms.iums.ac.ir/browse.php?a_code=A-10-7914-1&slc_lang=fa&sid=1 Kazem Talebi کاظم طالبی 3900319475328460089048 3900319475328460089048 No PhD Student, Department of Physical Education, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran دانشجوی دکترای تربیت بدنی، گروه تربیت بدنی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران Rambod Khajei رامبد خواجه ای R.khajeie@gmail.com 3900319475328460089049 3900319475328460089049 Yes Department of Physical Education, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran گروه تربیت بدنی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران Mohammad Reza Ramezanpour محمدرضا رمضانپور 3900319475328460089050 3900319475328460089050 No Department of Physical Education, Mashhad Branch, Islamic Azad University, Mashhad, Iran گروه تربیت بدنی و علوم ورزشی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران Ameneh Borjaste Yazdi آمنه برجسته یزدی 3900319475328460089051 3900319475328460089051 No Department of Physical Education, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran گروه تربیت بدنی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران Amir Rashid Lamir امیر رشید لمیر 3900319475328460089052 3900319475328460089052 No Department of Physical Physiology, Sport Science Faculty, Ferdowsi University, Mashhad, Iran گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه فردوسی مشهد، مشهد، ایران