Razi Journal of Medical Sciences

fa تاثیر سه ماه تمرین هوازی اینتروال و مکمل کوئرستین بر بیان پروتئین BDNF در هیپوکامپ موش‌های صحرایی نر مدل پارکینسونی The effect of three months of interval aerobic exercise and quercetin supplementation on BDNF protein expression in the hippocampus of male Parkinsonian model rats فیزیولوژی ورزش Exercise Physiology پژوهشي Research <div style="text-align: justify;">زمینه و هدف: بیماری پارکینسون پس از آلزایمر شایعترین بیماری تخریب نورونی رایج در جهان است. هدف از انجام تحقیق حاضر تبیین تاثیر سه ماه تمرین هوازی اینتروال و مکمل کوئرستین بر بیان پروتئین BDNF در هیپوکامپ موش‌های صحرایی نر مدل پارکینسونی بود. روششناسی: برای انجام تحقیق آزمایشی حاضر 28 سر رت نر نژاد ویستار 10-8 هفته ای و با وزن بدن 200-180 گرمی تهیه و به 5 گروه کنترل سالم 5 سر، کنترل پارکینسونی 5 سر، تمرین پارکینسونی: 6 سر ، ملاتونین پارکینسونی: 6 سر و تمرین + مکمل پارکینسونی: 6 سر) تقسیم شدند. سپس گروههای تمرین به مدت 12 هفته برنامه تمرین اینتروال را انجام داده و روزانه 100 میلی گرم بر کیلوگرم وزن بدن کوئرستین مصرف کردند. در نهایت و بعد از آخرین جلسه تمرینی بافت هیپوکامپ جهت بررسی متغیرهای تحقیق برداشته شد. نتایج: نتایج نشان داد اثر متقابل تمرین و مکمل از یک طرف و اثر اصلی تمرین از طرف دیگر بر BDNF معنادار نیست اما اثر اصلی مکمل کروئستین بر BDNF در رتهای مبتلا به پارکینسون معنادار است. نتیجهگیری: با توجه به نتایج استفاده از تمرین هوازی اینتورال و مکمل کروئسین برای بیماران پارکینسونی زیر نظر متخصص توصیه می شود.  </div> <pre style="text-align:justify"> 1.Introduction </pre> <pre style="text-align:justify"> Parkinson's disease is a debilitating disease that is widespread in people over 50 years of age and is the most common neurodegenerative disease in the world after Alzheimer's (1). Most aspects of the life of affected people are affected, and most patients complain of walking, getting up, lack of energy, the need to spend extra energy to do daily tasks, and also non-motor symptoms such as defects in smell, memory and digestion. (2). Brain protective protein or brain-derived neurotrophic factor (BDNF), which prevents the destruction of dopaminergic neurons, this factor plays a vital role in the development and maintenance of the health of the central and peripheral nervous system, the plasticity of synapses and brain neurons, proliferation and It is responsible for nerve cell survival as well as learning, memory and mood changes (12). Alpha-synuclein is a highly conserved protein that is abundant in the central nervous system (CNS) (14). Alpha-synuclein naturally exists in a folded state in the cytoplasm of the cell, which lacks secondary regular structures and has a random twist structure (15). The structure of the protein is changed in connection with lipid membrane structures (16).</pre> Considering the above information and the negative impact of Parkinson's disease on personal and social life on the one hand, the importance and impact of physical activity on Parkinson's disease on the other hand, as well as having different properties of quercetin, and finally considering that the researchers still have a general consensus about the best The training method alone or in combination with a certain type of medicinal plants have not been found, also due to the lack of research on the simultaneous effect of interval training and quercetin on its research variables in parkinsonian patients, the researchers are trying to answer the question whether Does interval aerobic exercise and quercetin supplementation affect the expression of BDNF and alpha-synuclein proteins in the hippocampus of male Parkinsonian model rats? 2.Methods <pre style="text-align:justify"> </pre> <pre style="text-align:justify"> The current research is experimental and applied. To do that, 28 male Wistar rats, 8-10 weeks old and weighing 180-200 grams, after 2 weeks of familiarization with the environment, were randomly divided into five groups of healthy rats, which were killed and operated at the end of the protocol, and training and did not use supplements (5 head healthy reference group), Parkinsonian rats that were killed and operated at the end of the protocol and did not exercise or take supplements (5 heads Parkinsonian control group), Parkinsonian rats that were killed and operated at the end of the protocol and only participated in interval aerobic exercise (Parkinson's exercise group: 6 subjects), Parkinsonian rats that were killed and operated on at the end of the protocol and took only supplements (Parkinson's melatonin group: 6 heads) and Parkinsonian rats that were killed and operated on at the end of the protocol and participated in both interval aerobic exercise and supplementation ( Exercise group + Parkinson's supplement: 6 heads) were divided. Next, the training groups performed the aerobic training program according to the following protocol:</pre> To perform the aerobic interval activity, rats were first introduced to the five-rail treadmill (Azma technique, made in Iran) for one week to reduce the stress of the new environment. Also, the intensity of the activity in the first two weeks of training started from 10 meters per minute for 15 minutes, then the duration of the activity reached 30 minutes. The incline of the treadmill was fixed at 5 degrees from the beginning to the end of the training period. For each training session, 5 minutes of warm-up at a speed of 5-10 m/min and the same amount of cool-down were considered. (30) The protocol of aerobic training was for 12 weeks and 5 sessions per week. The details of the protocol, including speed, time and incline, were determined each week after performing the exercise capacity test and determining VO2peak. It should be noted that the supplement groups also consumed quercetin supplement for 12 weeks orally and daily at 100 mg/kg of body weight manufactured by Sigma Company in the form of water solution, at 8 to 10 in the morning. Finally, immediately after the last training session, all the rats were anesthetized using chloroform and the hippocampal tissue of their brains was removed to check the research variables. After descriptive statistics and Shapiro-Wilk tests, two-way analysis of variance and Tukey's follow-up were used for statistical analysis of the data. 3.Results In order to explain the effect of exercise and crostinin supplementation on BDNF protein level in rats with Parkinson's disease, two-way analysis of variance test was used and the results showed that the interaction effect between exercise and supplementation on BDNF is not significant (F1=0.24). 655 and P=0.426) It was also found that the main effect of exercise on BDNF is not significant (F1,24=653.3 and P=0.068), but the main effect of croestin supplementation on BDNF is significant (F1,24 =5.093 and P=0.033). Considering the significance of the effect of the supplement, Bonferroni's post hoc test was used to compare the groups and the results showed that there is a significant difference between supplement use and non-supplement use. 4.Discussion <pre style="text-align:justify"> The results of the present study showed that the interaction effect of exercise and supplementation and the main effect of exercise on BDNF are not significant. However, it was found that the main effect of croestin supplement on BDNF is significant. In this regard, researches have shown that the prescription of food programs that contain natural antioxidants (such as fruits, plants, legumes, oil seeds, whole grains and vegetables, as well as the consumption of foods processed with Natural antioxidants such as vitamin C and E, carotenoids and polyphenols) can provide a suitable antioxidant status and help prevent neurodegenerative and metabolic disorders. On the other hand, it has been found that BDNF is one of the most important neurotrophins that is widely distributed in the central nervous system and has a significant effect on memory and learning functions (36). On the other hand, it has been proven that quercetin is a type of flavonol that is found naturally in most fruits, vegetables, leaves and seeds, and also its varieties in the form of food supplements and consumption supplements. There is. This substance has antioxidant, anti-cancer, anti-viral, and anti-inflammatory properties. Also, quercetin fights free radicals and helps to strengthen the flow of nutrients in the blood vessels (38). Probably the reason for the effect of quercetin on BDNF in the present study is due to the effect of quercetin's antioxidant properties. However, in the present study, it was found that the interaction effect of exercise and croestin supplementation on BDNF in rats with Parkinson's disease is not significant, which is probably due to the higher pressure of exercise and the production of ROS and free radicals.</pre> <pre style="text-align:justify"> </pre> تمرین هوازی اینتروال, کوئرستین, هیپوکامپ, BDNF Interval aerobic exercise, quercetin, hippocampus, BDNF 1 11 http://rjms.iums.ac.ir/browse.php?a_code=A-10-7431-1&slc_lang=fa&sid=1 Sirous Kianmehr سیروس کیان مهر sirous.kianmehr@yahoo.com 3900319475328460090639 3900319475328460090639 No Islamic Azad University دانشگاه آزاد تبریز Jabbar Bashiri جبار بشیری bashiri.jabbar@iaut.ac.ir 3900319475328460090640 3900319475328460090640 Yes Islamic Azad University دانشگاه آزاد تبریز Roghayeh Pouzesh Jadidi رقیه پوزش جدیدی poozesh2016@gmail.com 3900319475328460090641 3900319475328460090641 No Islamic Azad University دانشگاه آزاد تبریز Hassan Pourrazi حسن پوررضی pourrazi@soc.ikiu.ac.ir 3900319475328460090642 3900319475328460090642 No Imam Khomeini International University دانشگاه بین المللی امام خمینی MirAli NourAzar میرعلی نورآذر noura347@yahoo.com 3900319475328460090643 3900319475328460090643 No Islamic Azad University دانشگاه آزاد تبریز