Razi Journal of Medical Sciences
مجله علوم پزشکی رازی
RJMS
Medical Sciences
http://rjms.iums.ac.ir
39
journal39
2228-7043
2228-7051
en
jalali
1403
12
1
gregorian
2025
3
1
32
1
online
1
fulltext
fa
پاسخ عوامل نوروالتهابی مغز در رت های مبتلا به آنسفالومیلیت خود ایمن تجربی به اثر حفاظتی تمرینات اینتروال و مصرف ویتامین D
The response of Neuro-Inflammatory Factors in brain Tissue in rats with experimental autoimmune encephalomyelitis to the protective effect of interval training and vitamin D
فیزیولوژی ورزش
Exercise Physiology
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">زمینه و هدف: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">مولتیپل اسکلروزیس یک بیماری خودایمن با ضایعات ملتهب و دمیلینیه شونده در سیستم عصبی مرکزی می­باشد. هدف از این مطالعه بررسی پاسخ عوامل نوروالتهابی مغز در رت های مبتلا به آنسفالومیلیت خود ایمن تجربی به اثر حفاظتی تمرینات اینتروال و مصرف ویتامین </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">D</span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> بود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">مواد و روش</span></span></span></b><b><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">­</span></span></span></b><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">ها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> در این پژوهش تجربی، </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">48سر</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">موش</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">ماده</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">نژاد</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">لوئیز</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">4هفته­ای</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">به</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">­</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">طور</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">تصادفی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">به</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">8گروه</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">تقسیم</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">شدند. </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">پروتکل اینتروال شامل۱۰ تکرار ۱ دقیقه</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">­</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">ای، نسبت کار به استراحت ۱ به ۲ و کل زمان دویدن ۳۰دقیقه بود. شدت تمرین به تدریج از ۲۵ متر در دقیقه به ۷۰متر در دقیقه افزایش یافت. </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">رت­ها با نخاع خوکچه هندی و ادجوانت کامل ایمونیزه شدند. مدل </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">EAE</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> در انتهای هفته ششم تمرینات القاء شد</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">. رت­ها</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">به</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">ازای</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">هر</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">کیلوگرم وزن</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">بدن</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">5میکروگرم</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">ویتامین </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> D3</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">در</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">150میکرولیتر</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">روغن کنجد</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">هر</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">دو</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">روز</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">یک</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">بار به مدت 2هفته</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">به­صورت</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">داخل صفاقی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">دریافت کردند.</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">داده</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">­</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">ها به روش آنالیز واریانس یک</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">­</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">طرفه و آزمون تعقیبی توکی در سطح معنی</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">­</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">داری </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">p<0.05</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> تجزیه و تحلیل شد. </span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="tab-stops:right 446.45pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">نتایج:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">نتایج نشان داد که بین گروه­ها در شاخص </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">NF-KB</span><span b="" style="font-family:" zar=""> و </span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">TGF-B</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">تفاوت معنی­داری وجود </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">نداشت (05/0</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">p></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">). میانگین تغییرات </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">IL-17</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> در گروه ویتامین </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">D</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> در مدل </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">EAE</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> افزایش معنی­داری نسبت به گروه کنترل سالم ویتامین </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">D</span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> داشت (01/0=</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">). </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">میانگین تغییرات </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">GM-CSF</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">در گروه­های</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">اینتروال سالم (018/0=</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">)، </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">D</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> کنترل سالم (02/0=</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">)، </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">D</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> اینتروال سالم(001/0=</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">) و </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">D</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> اینتروال</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">MS </span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> (03/0=</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">) </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">کاهش معنی­داری نسبت به گروه کنترل سالم </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">داشت</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">. </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">نتیجه­گیری:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">تمرینات اینتروال </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">و ویتامین با بهبود </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">عوامل نوروالتهابی مغز در رت های مبتلا به آنسفالومیلیت خود ایمن تجربی همراه نبود. </span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Background: </span></span></span></b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Multiple sclerosis is an autoimmune disease with inflammatory and demyelinating lesions in the central nervous system.<b> </b>The aim of this study was to assessing the response of Neuro-Inflammatory Factors in brain Tissue in rats with experimental autoimmune encephalomyelitis to the protective effect of interval training and vitamin D.</span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Material and method</span></span></span></b><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> In this experimental study, 48 male Louise 4-week-old female rats were randomly divided into 8 groups. The interval protocol consisted of 10 repetitions of 1 minute, the ratio of work to rest was 1:2, and the total running time was 30 minutes. The training intensity was gradually increased from 25 meters per minute to 70 meters per minute. The rats were immunized with the guinea pig's spit and complete adjuvant. The EAE model was induced at the end of the sixth week of exercise. Rats received 5 μg of body weight per kilogram of vitamin D3 in 150 μl of sesame oil every two days once for 2 weeks’ intraperitoneal injection. Data were analyzed by one-way analysis of variance and Tukey post hoc test at the p <0.05.</span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Results: </span></span></span></b><span lang="EN" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">The results showed that there was no significant difference between the groups in NF-KB and TGF-B (p>0.05). The average changes of IL-17 in the vitamin D group in the EAE model had significant increase compared to the healthy vitamin D control group (P=0.01). The average changes of GM-CSF in healthy interval groups (P=0.018), D healthy control (P=0.02), D healthy interval (P=0.001) and D interval MS (P=0.03) There was significant decrease compared to the healthy control group.</span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Lotus""></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Conclusion: </span></span></span></b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">interval training and </span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">vitamin D</span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> were not associated with the improvement of brain neuroinflammatory factors in rats with experimental autoimmune encephalomyelitis.</span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Lotus""></span></span></span></span></span></span><br>
مولتیپل اسکلروزیس, تمرین اینتروال, ویتامین D, عوامل نوروالتهابی
Multiple Sclerosis, interval Training, Vitamin D, Neuro-Inflammatory Factors
1
10
http://rjms.iums.ac.ir/browse.php?a_code=A-10-7165-1&slc_lang=fa&sid=1
Smatalsadat
Mousavi
عصمت السادات
موسوی
Falahmohammadi47@gmail.com
3900319475328460090570
3900319475328460090570
No
Farhangian University, Nasibah Campus, Tehran,
فیزیولوژی ورزشی، دانشگاه فرهنگیان، پردیس نسیبه، تهران، ایران
Zia
Falahmohammadi
ضیاء
فلاح محمدی
mousavi2753@gmail.com
3900319475328460090571
3900319475328460090571
Yes
Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Mazandaran, Iran
گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه مازندران، بابلسر، ایران.