Razi Journal of Medical Sciences

fa تاثیرتمرین هوازی و مصرف عصاره آناناس بر بیان ژن P53 بافت کبد و حجم تومور موش های مبتلا به سرطان ملانوما The Effect of Aerobic Exercise and Pineapple Extract Consumption on Liver Tissue P53 Gene Expression and Tumor Volume in Mice with Melanoma Cancer فیزیولوژی ورزش Exercise Physiology پژوهشي Research <div style="text-align: justify;">زمینه و هدف: سرطان ملانوما شدیدترین سرطان پوست است. اخیراً نقش تمرینات ورزشی در پیشگیری و درمان سرطان بسیار مورد توجه قرار دارد. آناناس میوه‌ای است که خواص ضد سرطانی آن مطرح شده است.P53  یک ژن مهار کننده تومور است. هدف پژوهش حاضر مطالعه تاثیر تمرین هوازی و مصرف عصاره آناناس بر بیان ژن P53 بافت کبد و حجم تومور پس از انجام تمرین هوازی و مصرف عصاره آناناس در موش‌های سرطانی بود. روش کار: این مطالعه بنیادی بر روی  32 سر موش های نژاد C57  انجام شد. پس از کشت رده سلولی سرطان ملانوما القای تومور به موش ها صورت گرفت و پروتکل تمرین هوازی و گاواژ بر روی آن ها در چهار گروه شامل کنترل، تمرین هوازی، عصاره آناناس و تمرین هوازی- آناناس انجام شد. برنامه تمرین هوازی به مدت شش هفته انجام شد و عصاره آناناس به میزان 300 میلی گرم/کیلوگرم گاواژ شد. وزن با ترازوی دیجیتال و حجم تومور موش ها با استفاده از کولیس اندازه گیری شد. 48 ساعت پس از آخرین جلسه تمرین، نمونه گیری خونی و  بافت کبد انجام شد ، بیان ژن P53 بافت کبد به روش RT-PCR انجام گرفت. سپس داده ها با استفاده آنالیز واریانس یک طرفه، آزمون تحلیل واریانس دو عاملی و تعقیبی مورد تجزیه تحلیل قرار گرفتند و سطح معنی داری 05/0  p≤ در نظر گرفته شد. یافته‌ها: در مقایسه با گروه کنترل، تمرین هوازی و تمرین - آناناس به کاهش معنیدار حجم تومور و افزایش بیان ژن P53 بافت کبدی منجر شد. نتیجه‌گیری: ورزش هوازی و عصاره آناناس منجر به کاهش وزن وکاهش معنی‌دارحجم تومور و افزایش بیان ژن p53 سرکوب‌کننده تومور در گروه‌های تجربی نسبت به گروه کنترل در بافت کبد شد. </div> <div style="text-align: justify;"> Bakcground & Aims: Melanoma is the most severe subset of skin cancer that has high invasive power and rapid metastasis to other organs. Recently, the role of exercise in the prevention and treatment of cancer has received much attention. Pineapple belongs to the bromoliaceae family and the subfamily Bromolyidae, which is one of the most popular tropical fruits, including protein, fat, sugars, calcium, phosphorus, iron, sodium, potassium, thiamine, riboflavin, niacin, enzyme, bromilinil. Organic free is vitamin C, vitamin A, B, whose anti-cancer properties have been proposed. P53 is a tumor-inhibiting gene that is more associated with cancer. The aim of this study was to study the changes in liver tissue p53 gene expression and tumor volume after aerobic exercise and pineapple extract consumption in mic with melanoma cancer. Methods: This study was performed on 32 C57 mice (Weight: 12-14gr) .After culture of melanoma cancer cell line, tumor induction was performed in mice and aerobic training and gavage protocols were performed on them in four groups including control, aerobic training, pineapple extract and aerobic-pineapple training. The aerobic exercise program was performed for six weeks with the treadmill. Familiarization protocol was performed at a speed of 6-10 meters per minute for tow week. The continuous training program started at 14 meters per minute in the first two weeks and finally reached 16 meters per minute in the last two weeks. In order to eliminate the effect of stress on the studied variables, control mice were also placed on the treadmill for the duration of the exercise program for 20 minutes.pineapple extract was gavage at 300 mg / kg. Weight was measured with a digital scale and tumor volume of mice using a caliper. 48 hours after the last training session, blood sampling and liver tissue were performed, expression of p53 gene of liver tissue was performed by RT-PCR. Then, the data were analyzed using one-way analysis of variance, two-factor analysis of variance and post hoc, and a significance level of p≤0.05 was considered.The above research has the code of ethics IR.IAU.SRB.REC.1399.178 in the Medical Ethics Committee of the Azad University, Science and Research Branch. Results: The results showed that compared to the control group, aerobic exercise and pineapple exercise significantly reduced tumor volume and increased expression of hepatic p53 gene. Levels of variables including mice weight, tumor volume, P53 gene, mice as mean +/- standard deviation are reported. Results shows the results of two-factor analysis of variance and Bonferroni post hoc as well as the effect size of the groups. Conclusion: The findings of the present study showed that aerobic exercise and pineapple extract resulted in weight loss and significant reduction in tumor volume and increased expression of p53 tumor suppressor gene in experimental groups compared to controls in liver tissue. The results showed that the weight of mice was lower in the experimental groups than in the control group, while in the training-pineapple group, the weight loss was higher. The results also showed that the tumor volume decreased in the experimental groups, which was significant in the pineapple and interactive exercise-pineapple groups, and in the exercise group, there was a non-significant decrease in the tumor volume of mice with melanoma cancer. With melanoma further reduced the size of the effect of pineapple extract. The results also showed that the changes in tumor volume between the exercise-pineapple and control groups showed a greater decrease than the other groups, which is significant, and in the other groups there is a significant decrease compared to the control group. These results indicate that different types of exercise can play an effective role in suppressing tumor growth. All of these findings emphasize the anti-inflammatory effects of exercise training in mice with cancer, which can also lead to a significant reduction in tumor volume. The results of the present study also showed that the expression of p53 tumor suppressor gene was significantly increased in the experimental groups, which was higher in the exercise and exercise-pineapple groups. The importance of autonomic functions of p53 tumor suppressor cancer cells (encoded by TP53) has been identified in many studies, but it is now clear that the status of p53 cancer cells also has a significant effect on the immune response. The most obvious result of the loss or mutation of p53 in mice and humans is the increased development of cancer. It is clear that the main function of p53 in humans is to protect against the progression of malignancy, but other functions of p53 are the ability to regulate immune responses and their role in the pathology of non-cancerous diseases, including wound healing, wound healing and wound healing or viral infection control. The mechanisms regulated by P53 also regulate mitochondrial respiration and help maintain genomic stability. Studies show that improving aerobic metabolism by P53, which acts as a tumor suppressor, may provide future cancer prevention strategies. Since herbal compounds have fewer side effects compared to chemical compounds, identifying new strategies for using medicinal plants as a suitable alternative to chemical drugs along with proper exercise can help cancer patients recover.               </div> تمرین هوازی, عصاره آناناس , سرطان ملانوما, کبد ,P53 Aerobic exercise, Pineapple, Melanoma cancer, Liver 1 13 http://rjms.iums.ac.ir/browse.php?a_code=A-10-6713-1&slc_lang=fa&sid=1 Hamideh Tavoli حمیده طاولی 3900319475328460089535 3900319475328460089535 No PhD Student of Exercise Physiology, department of Physical Education and Sport Science, Science and Research Branch, Islamic Azad University, Tehran, Iran دانشجوی دکتری فیزیولوژی ورزشی، گروه تخصصی تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران Hossein Abed natanzi حسین عابد نطنزی abednazari@gmail.com 3900319475328460089536 3900319475328460089536 Yes Department of Physical Education and Sport Science, Science and Research Branch, Islamic Azad University, Tehran, Iran گروه تخصصی تربیت بدنی و علوم ورزشی،دانشگاه آزاد اسلامی،واحد علوم و تحقیقات، تهران، ایران Mohammad Ali Azarbayjani محمدعلی آذربایجانی 3900319475328460089537 3900319475328460089537 No Department of Exercise Physiology, Sport Medicine Research Center, Sport Sciences Research Institute, Tehran, Iran استاد گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی تهران مرکزی، تهران، ایران Alireza Iranbakhsh علیرضا ایرانبخش 3900319475328460089538 3900319475328460089538 No Department of Physical Education and Sport Science, Science and Research Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، دانشکده:علوم و فناوری‌های همگرا، دانشگاه آزاد اسلامی،واحد علوم و تحقیقات، تهران، ایران